PRV-1, erythroid colonies and platelet Mpl are unrelated to thrombosis in essential thrombocythaemia.

TY - JOUR

T1 - PRV-1, erythroid colonies and platelet Mpl are unrelated to thrombosis in essential thrombocythaemia.

AU - Vannucchi, Alessandro M

AU - Pancrazzi, Alessandro

AU - Antonioli, Elisabetta

AU - Guglielmelli, Paola

AU - Balestri, Francesca

AU - Biscardi, Monica

AU - Bulgarelli, Simona

AU - Longo, Giovanni

AU - Graziano, Claudio

AU - Gugliotta, Luigi

AU - Bosi, Alberto

N1 - Keywords: Adult; Blood Platelets; Clone Cells; Dosage Compensation, Genetic; Erythroid Progenitor Cells; Female; Gene Expression; Humans; Isoantigens; Logistic Models; Membrane Glycoproteins; Middle Aged; Myelopoiesis; Predictive Value of Tests; Receptors, Cell Surface; Thrombocythemia, Hemorrhagic; Thrombopoietin; Thrombosis

PY - 2004

Y1 - 2004

N2 - Females with the monoclonal type of essential thrombocythaemia (ET), based on the X-chromosome inactivation pattern (XCIP), have previously been shown to present a higher incidence of thrombosis than polyclonal ones. We aimed to assess correlations between XCIP, thrombosis, and three epigenetic markers of ET, namely PRV-1 overexpression, endogenous erythroid colony (EEC) formation, and reduced platelet Mpl content. Fifty-three (60%) of 88 subjects studied had monoclonal myelopoiesis and presented a 32% incidence of major thrombosis compared with 6% of polyclonal subjects (P = 0.009). The frequency of abnormalities of PRV-1, EEC, or Mpl was similar in monoclonal and polyclonal subjects (respectively, 28%, 48%, 75%, and 37%, 27%, 63%), and none of them correlated with thrombosis. We conclude that the exploited epigenetic markers constitute independent phenotypic variations and are not clustered according to monoclonality of myelopoiesis in ET; none of them could serve as a surrogate marker of thrombotic risk in male subjects with ET.

AB - Females with the monoclonal type of essential thrombocythaemia (ET), based on the X-chromosome inactivation pattern (XCIP), have previously been shown to present a higher incidence of thrombosis than polyclonal ones. We aimed to assess correlations between XCIP, thrombosis, and three epigenetic markers of ET, namely PRV-1 overexpression, endogenous erythroid colony (EEC) formation, and reduced platelet Mpl content. Fifty-three (60%) of 88 subjects studied had monoclonal myelopoiesis and presented a 32% incidence of major thrombosis compared with 6% of polyclonal subjects (P = 0.009). The frequency of abnormalities of PRV-1, EEC, or Mpl was similar in monoclonal and polyclonal subjects (respectively, 28%, 48%, 75%, and 37%, 27%, 63%), and none of them correlated with thrombosis. We conclude that the exploited epigenetic markers constitute independent phenotypic variations and are not clustered according to monoclonality of myelopoiesis in ET; none of them could serve as a surrogate marker of thrombotic risk in male subjects with ET.

U2 - 10.1111/j.1365-2141.2004.05175.x

DO - 10.1111/j.1365-2141.2004.05175.x

M3 - Journal article

C2 - 15461629

SN - 0963-1860

VL - 127

SP - 214

EP - 219

JO - British Journal of Haematology, Supplement

JF - British Journal of Haematology, Supplement

IS - 2

ER -