Proteins containing the UBA domain are able to bind to multi-ubiquitin chains.

C R Wilkinson; M Seeger; R Hartmann-Petersen; M Stone; M Wallace; C Semple; C Gordon

doi:10.1038/ncb1001-939

Proteins containing the UBA domain are able to bind to multi-ubiquitin chains.

C R Wilkinson, M Seeger, R Hartmann-Petersen, M Stone, M Wallace, C Semple, C Gordon

Biomolecular Sciences

337 Citations (Scopus)

Abstract

The UBA domain is a motif found in a variety of proteins, some of which are associated with the ubiquitin-proteasome system. We describe the isolation of a fission-yeast gene, mud1+, which encodes a UBA domain containing protein that is able to bind multi-ubiquitin chains. We show that the UBA domain is responsible for this activity. Two other proteins containing this motif, the fission-yeast homologues of Rad23 and Dsk2, are also shown to bind multi-ubiquitin chains via their UBA domains. These two proteins are implicated, along with the fission-yeast Pus1(S5a/Rpn10) subunit of the 26 S proteasome, in the recognition and turnover of substrates by this proteolytic complex.

Original language	English
Journal	Nature Cell Biology
Volume	3
Issue number	10
Pages (from-to)	939-43
Number of pages	4
ISSN	1465-7392
DOIs	https://doi.org/10.1038/ncb1001-939
Publication status	Published - 2001

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title = "Proteins containing the UBA domain are able to bind to multi-ubiquitin chains.",

abstract = "The UBA domain is a motif found in a variety of proteins, some of which are associated with the ubiquitin-proteasome system. We describe the isolation of a fission-yeast gene, mud1+, which encodes a UBA domain containing protein that is able to bind multi-ubiquitin chains. We show that the UBA domain is responsible for this activity. Two other proteins containing this motif, the fission-yeast homologues of Rad23 and Dsk2, are also shown to bind multi-ubiquitin chains via their UBA domains. These two proteins are implicated, along with the fission-yeast Pus1(S5a/Rpn10) subunit of the 26 S proteasome, in the recognition and turnover of substrates by this proteolytic complex.",

author = "Wilkinson, {C R} and M Seeger and R Hartmann-Petersen and M Stone and M Wallace and C Semple and C Gordon",

note = "Keywords: Amino Acid Motifs; Cell Cycle Proteins; Cysteine Endopeptidases; DNA-Binding Proteins; Fungal Proteins; Genes, Fungal; Genes, Reporter; Multienzyme Complexes; Phenotype; Proteasome Endopeptidase Complex; Protein Binding; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Surface Plasmon Resonance; Ubiquitin; Ubiquitins",

year = "2001",

doi = "10.1038/ncb1001-939",

language = "English",

volume = "3",

pages = "939--43",

journal = "Nature Cell Biology",

issn = "1465-7392",

publisher = "nature publishing group",

number = "10",

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TY - JOUR

T1 - Proteins containing the UBA domain are able to bind to multi-ubiquitin chains.

AU - Wilkinson, C R

AU - Seeger, M

AU - Hartmann-Petersen, R

AU - Stone, M

AU - Wallace, M

AU - Semple, C

AU - Gordon, C

N1 - Keywords: Amino Acid Motifs; Cell Cycle Proteins; Cysteine Endopeptidases; DNA-Binding Proteins; Fungal Proteins; Genes, Fungal; Genes, Reporter; Multienzyme Complexes; Phenotype; Proteasome Endopeptidase Complex; Protein Binding; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Surface Plasmon Resonance; Ubiquitin; Ubiquitins

PY - 2001

Y1 - 2001

N2 - The UBA domain is a motif found in a variety of proteins, some of which are associated with the ubiquitin-proteasome system. We describe the isolation of a fission-yeast gene, mud1+, which encodes a UBA domain containing protein that is able to bind multi-ubiquitin chains. We show that the UBA domain is responsible for this activity. Two other proteins containing this motif, the fission-yeast homologues of Rad23 and Dsk2, are also shown to bind multi-ubiquitin chains via their UBA domains. These two proteins are implicated, along with the fission-yeast Pus1(S5a/Rpn10) subunit of the 26 S proteasome, in the recognition and turnover of substrates by this proteolytic complex.

AB - The UBA domain is a motif found in a variety of proteins, some of which are associated with the ubiquitin-proteasome system. We describe the isolation of a fission-yeast gene, mud1+, which encodes a UBA domain containing protein that is able to bind multi-ubiquitin chains. We show that the UBA domain is responsible for this activity. Two other proteins containing this motif, the fission-yeast homologues of Rad23 and Dsk2, are also shown to bind multi-ubiquitin chains via their UBA domains. These two proteins are implicated, along with the fission-yeast Pus1(S5a/Rpn10) subunit of the 26 S proteasome, in the recognition and turnover of substrates by this proteolytic complex.

U2 - 10.1038/ncb1001-939

DO - 10.1038/ncb1001-939

M3 - Journal article

C2 - 11584278

SN - 1465-7392

VL - 3

SP - 939

EP - 943

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 10

ER -

Proteins containing the UBA domain are able to bind to multi-ubiquitin chains.

Abstract

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