Abstract
Scid mice transplanted with low numbers of syngeneic CD4+ T cells, develop a chronic and lethal inflammatory bowel disease (IBD) within 4-6 months. We have used in vivo 5-bromo2-deoxy-uridine (BrdU) labeling to assess the proliferation of lamina propria-derived CD4+ T cells in diseased scid mice. The hourly rate of renewal of colonic lamina propria CD4+ T cells in diseased mice was 7% compared with 1.5% in normal BALB/c control mice. Transplantation of scid mice with in vitro activated CD4+ T cells accelerated the disease onset and development in a cell dose-dependent fashion when compared with non-activated CD4+ T cells. In pulse-chase experiments it was shown that BrdU-labeled cells disappeared rapidly from the lamina propria of diseased mice. DNA analysis revealed that this was due to the presence of nearly four times as many apoptotic CD4+ T cells in diseased than in control mice. Further analyses showed that the apoptotic lamina propria CD4+ T cells were derived from cells having entered the cell cycle within the previous 8 h. These data clearly demonstrate that vigorous CD4+ T cell proliferation and death are involved throughout the course of IBD.
| Original language | English |
|---|---|
| Journal | European Journal of Immunology |
| Volume | 28 |
| Issue number | 11 |
| Pages (from-to) | 3655-63 |
| Number of pages | 9 |
| ISSN | 0014-2980 |
| DOIs | |
| Publication status | Published - 1 Nov 1998 |
Keywords
- Animals
- Apoptosis
- Bromodeoxyuridine
- CD4-Positive T-Lymphocytes
- Inflammatory Bowel Diseases
- Intestinal Mucosa
- Lymphocyte Activation
- Mice
- Mice, Inbred BALB C
- Mice, SCID
- Rats