Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells

Alexis de Ghellinck; Hubert Schaller; Valerie Laux; Michael Haertlein; Michele Sferrazza; Michele Sferrazza; Hanna Wacklin; Juliette Jouhet; Giovanna Fragneto

doi:10.1371/journal.pone.0092999

Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells

Alexis de Ghellinck, Hubert Schaller, Valerie Laux, Michael Haertlein, Michele Sferrazza, Michele Sferrazza, Hanna Wacklin, Juliette Jouhet, Giovanna Fragneto

Department of Chemistry

24 Citations (Scopus)

203 Downloads (Pure)

Abstract

Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes.

Original language	English
Article number	e92999
Journal	P L o S One
Volume	9
Issue number	4
Pages (from-to)	1-9
Number of pages	9
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0092999
Publication status	Published - 18 Apr 2014

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title = "Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells",

abstract = "Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes.",

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AU - de Ghellinck, Alexis

AU - Schaller, Hubert

AU - Laux, Valerie

AU - Haertlein, Michael

AU - Sferrazza, Michele

AU - Wacklin, Hanna

AU - Jouhet, Juliette

AU - Fragneto, Giovanna

PY - 2014/4/18

Y1 - 2014/4/18

N2 - Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes.

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Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells

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