Probing interactions between β-Glucan and bile salts at atomic detail by 1H-13C NMR assays

Mette Skau Mikkelsen; Sofia Bolvig Cornali; Morten G. Jensen; Mathias Nilsson; Sophie R. Beeren; Sebastian Meier

doi:10.1021/jf504352w

Probing interactions between β-Glucan and bile salts at atomic detail by ¹H-¹³C NMR assays

Mette Skau Mikkelsen, Sofia Bolvig Cornali, Morten G. Jensen, Mathias Nilsson, Sophie R. Beeren, Sebastian Meier

Dairy, Meat and Plant Product Technology

10 Citations (Scopus)

Abstract

Polysaccharides are prospective hosts for the delivery and sequestration of bioactive guest molecules. Polysaccharides of dietary fiber, specifically cereal (1 → 3)(1 → 4)-β-glucans, play a role in lowering the blood plasma cholesterol level in humans. Direct host-guest interactions between β-glucans and conjugated bile salts are among the possible molecular mechanisms explaining the hypocholesterolemic effects of β-glucans. The present study shows that ¹H-¹³C NMR assays on a time scale of minutes detect minute signal changes in both bile salts and β-glucans, thus indicating dynamic interactions between bile salts and β-glucans. Experiments are consistent with stronger interactions at pH 5.3 than at pH 6.5 in this in vitro assay. The changes in bile salt and β-glucan signals suggest a stabilization of bile salt micelles and concomitant conformational changes in β-glucans.

Original language	English
Journal	Journal of Agricultural and Food Chemistry
Volume	62
Issue number	47
Pages (from-to)	11472-11478
Number of pages	7
ISSN	0021-8561
DOIs	https://doi.org/10.1021/jf504352w
Publication status	Published - 26 Nov 2014

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title = "Probing interactions between β-Glucan and bile salts at atomic detail by 1H-13C NMR assays",

abstract = "Polysaccharides are prospective hosts for the delivery and sequestration of bioactive guest molecules. Polysaccharides of dietary fiber, specifically cereal (1 → 3)(1 → 4)-β-glucans, play a role in lowering the blood plasma cholesterol level in humans. Direct host-guest interactions between β-glucans and conjugated bile salts are among the possible molecular mechanisms explaining the hypocholesterolemic effects of β-glucans. The present study shows that 1H-13C NMR assays on a time scale of minutes detect minute signal changes in both bile salts and β-glucans, thus indicating dynamic interactions between bile salts and β-glucans. Experiments are consistent with stronger interactions at pH 5.3 than at pH 6.5 in this in vitro assay. The changes in bile salt and β-glucan signals suggest a stabilization of bile salt micelles and concomitant conformational changes in β-glucans.",

author = "Mikkelsen, {Mette Skau} and Cornali, {Sofia Bolvig} and Jensen, {Morten G.} and Mathias Nilsson and Beeren, {Sophie R.} and Sebastian Meier",

year = "2014",

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day = "26",

doi = "10.1021/jf504352w",

language = "English",

volume = "62",

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journal = "Journal of Agricultural and Food Chemistry",

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T1 - Probing interactions between β-Glucan and bile salts at atomic detail by 1H-13C NMR assays

AU - Mikkelsen, Mette Skau

AU - Cornali, Sofia Bolvig

AU - Jensen, Morten G.

AU - Nilsson, Mathias

AU - Beeren, Sophie R.

AU - Meier, Sebastian

PY - 2014/11/26

Y1 - 2014/11/26

N2 - Polysaccharides are prospective hosts for the delivery and sequestration of bioactive guest molecules. Polysaccharides of dietary fiber, specifically cereal (1 → 3)(1 → 4)-β-glucans, play a role in lowering the blood plasma cholesterol level in humans. Direct host-guest interactions between β-glucans and conjugated bile salts are among the possible molecular mechanisms explaining the hypocholesterolemic effects of β-glucans. The present study shows that 1H-13C NMR assays on a time scale of minutes detect minute signal changes in both bile salts and β-glucans, thus indicating dynamic interactions between bile salts and β-glucans. Experiments are consistent with stronger interactions at pH 5.3 than at pH 6.5 in this in vitro assay. The changes in bile salt and β-glucan signals suggest a stabilization of bile salt micelles and concomitant conformational changes in β-glucans.

AB - Polysaccharides are prospective hosts for the delivery and sequestration of bioactive guest molecules. Polysaccharides of dietary fiber, specifically cereal (1 → 3)(1 → 4)-β-glucans, play a role in lowering the blood plasma cholesterol level in humans. Direct host-guest interactions between β-glucans and conjugated bile salts are among the possible molecular mechanisms explaining the hypocholesterolemic effects of β-glucans. The present study shows that 1H-13C NMR assays on a time scale of minutes detect minute signal changes in both bile salts and β-glucans, thus indicating dynamic interactions between bile salts and β-glucans. Experiments are consistent with stronger interactions at pH 5.3 than at pH 6.5 in this in vitro assay. The changes in bile salt and β-glucan signals suggest a stabilization of bile salt micelles and concomitant conformational changes in β-glucans.

U2 - 10.1021/jf504352w

DO - 10.1021/jf504352w

M3 - Journal article

C2 - 25375023

SN - 0021-8561

VL - 62

SP - 11472

EP - 11478

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 47

ER -

Probing interactions between β-Glucan and bile salts at atomic detail by 1H-13C NMR assays

Abstract

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Probing interactions between β-Glucan and bile salts at atomic detail by ¹H-¹³C NMR assays