Prediction of major histocompatibility complex binding regions of protein antigens by sequence pattern analysis

TY - JOUR

T1 - Prediction of major histocompatibility complex binding regions of protein antigens by sequence pattern analysis

AU - Sette, A

AU - Buus, S

AU - Appella, E

AU - Smith, J A

AU - Chesnut, R

AU - Miles, C

AU - Colon, S M

AU - Grey, H M

N1 - Keywords: Amino Acid Sequence; Animals; Antigens; Binding Sites; Eukaryotic Cells; Haplotypes; Histocompatibility Antigens Class II; Mice; Molecular Sequence Data; Ovalbumin; Prokaryotic Cells; Proteins; Viral Proteins

PY - 1989

Y1 - 1989

N2 - We have previously experimentally analyzed the structural requirements for interaction between peptide antigens and mouse major histocompatibility complex (MHC) molecules of the d haplotype. We describe here two procedures devised to predict specifically the capacity of peptide molecules to interact with these MHC class II molecules (IAd and IEd). The accuracy of these procedures has been tested on a large panel of synthetic peptides of eukaryotic, prokaryotic, and viral origin, and also on a set of overlapping peptides encompassing the entire staphylococcal nuclease molecule. For both sets of peptides, IAd and IEd binding was successfully predicted in approximately 75% of the cases. This suggests that definition of such sequence "motifs" could be of general use in predicting potentially immunogenic peptide regions within proteins.

AB - We have previously experimentally analyzed the structural requirements for interaction between peptide antigens and mouse major histocompatibility complex (MHC) molecules of the d haplotype. We describe here two procedures devised to predict specifically the capacity of peptide molecules to interact with these MHC class II molecules (IAd and IEd). The accuracy of these procedures has been tested on a large panel of synthetic peptides of eukaryotic, prokaryotic, and viral origin, and also on a set of overlapping peptides encompassing the entire staphylococcal nuclease molecule. For both sets of peptides, IAd and IEd binding was successfully predicted in approximately 75% of the cases. This suggests that definition of such sequence "motifs" could be of general use in predicting potentially immunogenic peptide regions within proteins.

M3 - Journal article

C2 - 2717617

SN - 0027-8424

VL - 86

SP - 3296

EP - 3300

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 9

ER -