On the regulatory functions of neuropeptide Y (NPY) with respect to vascular resistance and exocrine and endocrine secretion in the pig pancreas

TY - JOUR

T1 - On the regulatory functions of neuropeptide Y (NPY) with respect to vascular resistance and exocrine and endocrine secretion in the pig pancreas

AU - Holst, J J

AU - Orskov, C

AU - Knuhtsen, S

AU - Sheikh, S

AU - Nielsen, O V

PY - 1989/8

Y1 - 1989/8

N2 - We compared the effects of electrical stimulation of the splanchnic nerves and infusions of neuropeptide Y, noradrenaline or a combination of the two on pancreatic vascular resistance and exocrine and endocrine secretion. For these studies we used isolated perfused pig pancreas with preserved splanchnic nerve supply. The exocrine secretion was stimulated with physiological concentrations of secretin and cholecystokinin octapeptide. Noradrenaline and NPY at 10(-8) M both increased pancreatic perfusion pressure. Their effects were additive and similar in magnitude to that of electrical stimulation of the splanchnic nerves at 4-8 Hz. Nerve stimulation as well as NPY and noradrenaline infusions inhibited exocrine secretion, but an additive effect could not be demonstrated. Neither NPY nor noradrenaline could reproduce the stimulatory effect of nerve stimulation on glucagon secretion, nor the weak inhibitory effect on somatostatin secretion. NPY alone had no effect on insulin secretion and did not influence the inhibitory effect of noradrenaline. It is concluded that NPY is likely to cooperate with noradrenaline in the control of pancreatic blood flow, whereas its role in the control of pancreatic secretion is likely to be of minor importance, if any.

AB - We compared the effects of electrical stimulation of the splanchnic nerves and infusions of neuropeptide Y, noradrenaline or a combination of the two on pancreatic vascular resistance and exocrine and endocrine secretion. For these studies we used isolated perfused pig pancreas with preserved splanchnic nerve supply. The exocrine secretion was stimulated with physiological concentrations of secretin and cholecystokinin octapeptide. Noradrenaline and NPY at 10(-8) M both increased pancreatic perfusion pressure. Their effects were additive and similar in magnitude to that of electrical stimulation of the splanchnic nerves at 4-8 Hz. Nerve stimulation as well as NPY and noradrenaline infusions inhibited exocrine secretion, but an additive effect could not be demonstrated. Neither NPY nor noradrenaline could reproduce the stimulatory effect of nerve stimulation on glucagon secretion, nor the weak inhibitory effect on somatostatin secretion. NPY alone had no effect on insulin secretion and did not influence the inhibitory effect of noradrenaline. It is concluded that NPY is likely to cooperate with noradrenaline in the control of pancreatic blood flow, whereas its role in the control of pancreatic secretion is likely to be of minor importance, if any.

KW - Animals

KW - Cholecystokinin/administration & dosage

KW - Dose-Response Relationship, Drug

KW - Electric Stimulation

KW - Infusions, Intravenous

KW - Islets of Langerhans/drug effects

KW - Neuropeptide Y/administration & dosage

KW - Norepinephrine/administration & dosage

KW - Pancreas/drug effects

KW - Secretin/administration & dosage

KW - Splanchnic Nerves/physiology

KW - Swine

KW - Vascular Resistance/drug effects

U2 - 10.1111/j.1748-1716.1989.tb08697.x

DO - 10.1111/j.1748-1716.1989.tb08697.x

M3 - Journal article

C2 - 2675536

SN - 0001-6772

VL - 136

SP - 519

EP - 526

JO - Acta Physiologica Scandinavica

JF - Acta Physiologica Scandinavica

IS - 4

ER -