NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs

TY - JOUR

T1 - NMR-based metabonomics reveals that plasma betaine increases upon intake of high-fiber rye buns in hypercholesterolemic pigs

AU - Bertram, Hanne Christine S.

AU - Malmendal, Anders

AU - Nielsen, Niels Chr

AU - Straadt, Ida Krestine

AU - Larsen, Torben

AU - Knudsen, Knud Erik Bach

AU - Laerke, Helle Nygaard

PY - 2009

Y1 - 2009

N2 - This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.

AB - This study presents an NMR-based metabonomic approach to explore the overall endogenous biochemical effects of a rye versus wheat-based fiber-rich diet in hypercholesterolemic pigs. The pigs were fed high-fat, high-cholesterol rye- (n = 9) or wheat- (n = 8) based buns with similar levels of dietary fiber for 9-10 wk. Fasting plasma samples were collected 2 days before and after 8 and 12 days on the experimental diets, while postprandial samples taken after 58-67 days, and( 1)H NMR spectra were acquired on these. Principal component analysis on the obtained NMR spectra demonstrated clear effects of diet on the plasma metabolite profile, and partial least squares regression discriminant analysis on the spectra revealed that the intensity of the spectral region at 3.29 ppm dominated the differentiation between the two diets, as the rye diet was associated with higher spectral intensity in this region. The 3.29 ppm signal is ascribed to N(CH(3))(3) protons in betaine, which may be an important contributor to the health promoting effects of rye.

KW - Animals

KW - Betaine

KW - Cholesterol

KW - Female

KW - Magnetic Resonance Spectroscopy

KW - Metabolomics

KW - Secale cereale

KW - Swine

U2 - 10.1002/mnfr.200800344

DO - 10.1002/mnfr.200800344

M3 - Journal article

C2 - 19603403

SN - 1613-4125

VL - 53

SP - 1055

EP - 1062

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

IS - 8

ER -