Morphological and molecular evidence converge upon a robust phylogeny of the megadiverse Holometabola

Rolf Georg Beutel, Frank Friedrich, Thomas Hörnschemeyer, Hans Pohl, Frank Hünefeld, Felix Beckmann, Rudolf Meier, Bernard Misof, Michael F. Whiting, Lars Vilhelmsen

    101 Citations (Scopus)

    Abstract

    We present the largest morphological character set ever compiled for Holometabola. This was made possible through an optimized acquisition of data. Based on our analyses and recently published hypotheses based on molecular data, we discuss higher-level phylogeny and evolutionary changes. We comment on the information content of different character systems and discuss the role of morphology in the age of phylogenomics. Microcomputer tomography in combination with other techniques proved highly efficient for acquiring and documenting morphological data. Detailed anatomical information (356 characters) is now available for 30 representatives of all holometabolan orders. A combination of traditional and novel techniques complemented each other and rapidly provided reliable data. In addition, our approach facilitates documenting the anatomy of model organisms. Our results show little congruence with studies based on rRNA, but confirm most clades retrieved in a recent study based on nuclear genes: Holometabola excluding Hymenoptera, Coleopterida (=Strepsiptera+Coleoptera), Neuropterida excl. Neuroptera, and Mecoptera. Mecopterida (=Antliophora+Amphiesmenoptera) was retrieved only in Bayesian analyses. All orders except Megaloptera are monophyletic. Problems in the analyses are caused by taxa with numerous autapomorphies and/or inapplicable character states due to the loss of major structures (such as wings). Different factors have contributed to the evolutionary success of various holometabolan lineages. It is likely that good flying performance, the ability to occupy different habitats as larvae and adults, parasitism, liquid feeding, and co-evolution with flowering plants have played important roles. We argue that even in the "age of phylogenomics", comparative morphology will still play a vital role. In addition, morphology is essential for reconstructing major evolutionary transformations at the phenotypic level, for testing evolutionary scenarios, and for placing fossil taxa.

    Original languageEnglish
    JournalCladistics
    Volume27
    Issue number4
    Pages (from-to)341-355
    Number of pages15
    ISSN0748-3007
    DOIs
    Publication statusPublished - Aug 2011

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