Migratory, invasive and metastatic capacity of NCAM transfected rat glioma cells

TY - JOUR

T1 - Migratory, invasive and metastatic capacity of NCAM transfected rat glioma cells

AU - Edvardsen, K

AU - Brünner, N

AU - Spang-Thomsen, M

AU - Walsh, F S

AU - Bock, E

N1 - Keywords: Animals; Basement Membrane; Brain Neoplasms; Cell Adhesion Molecules, Neuronal; Cell Movement; Female; Fluorescent Antibody Technique; Glioma; Immunoblotting; Mice; Mice, Nude; Microscopy, Fluorescence; Neoplasm Invasiveness; Rats; Transfection; Tumor Cells, Cultured

PY - 1993

Y1 - 1993

N2 - A cDNA encoding a transmembrane 140 kDa isoform of the neural cell adhesion molecule, NCAM, was transfected into the rat glioma cell line BT4Cn. Transfectants with a homogeneously high expression of NCAM-B showed a decreased capacity for penetration of an artificial basement membrane when compared to cells transfected with expression-vector alone or untransfected cells. However, when injected subcutaneously into nude mice, both NCAM expressing cells and control cells produced invasive tumors. Nude mice injected with NCAM positive cells developed tumors with slower growth rates as compared to those induced by NCAM negative cells. This implies that NCAM may not only be involved in adhesive and motile behaviour of glioma cells, but also in their growth regulation.

AB - A cDNA encoding a transmembrane 140 kDa isoform of the neural cell adhesion molecule, NCAM, was transfected into the rat glioma cell line BT4Cn. Transfectants with a homogeneously high expression of NCAM-B showed a decreased capacity for penetration of an artificial basement membrane when compared to cells transfected with expression-vector alone or untransfected cells. However, when injected subcutaneously into nude mice, both NCAM expressing cells and control cells produced invasive tumors. Nude mice injected with NCAM positive cells developed tumors with slower growth rates as compared to those induced by NCAM negative cells. This implies that NCAM may not only be involved in adhesive and motile behaviour of glioma cells, but also in their growth regulation.

M3 - Journal article

C2 - 8116479

SN - 0736-5748

VL - 11

SP - 681

EP - 690

JO - International Journal of Developmental Neuroscience

JF - International Journal of Developmental Neuroscience

IS - 5

ER -