Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition

Annette Mouritsen; Tue Søeborg; Trine Holm Johannsen; Lise Aksglaede; Kaspar Sørensen; Casper P Hagen; Mikkel G Mieritz; Hanne Frederiksen; Anna-Maria Andersson; Anders Juul

doi:10.1159/000358560

Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition

Annette Mouritsen, Tue Søeborg, Trine Holm Johannsen, Lise Aksglaede, Kaspar Sørensen, Casper P Hagen, Mikkel G Mieritz, Hanne Frederiksen, Anna-Maria Andersson, Anders Juul

Department of Clinical Medicine

20 Citations (Scopus)

Abstract

BACKGROUND: Accurate and selective assessment of testosterone requires use of a sensitive LC-MS/MS method, especially at low levels as those seen in young children.

METHODS: The present longitudinal study of 20 healthy children from the Copenhagen Puberty Study followed every 6 months for 5 years evaluates the longitudinal increase in serum testosterone before, during and after pubertal onset quantified by a newly developed LC-MS/MS method in comparison with immunoassay. Testosterone concentrations in serum samples (n = 177) were determined by LC-MS/MS (detection limit 0.1 nmol/l) and by immunoassay (detection limit 0.23 nmol/l).

RESULTS: Serum concentrations of testosterone increased gradually with age by both methods. However, serum testosterone was quantifiable in 9/10 girls prior to pubic hair development measured with LC-MS/MS, and in 2/10 girls measured with immunoassay. In boys, testosterone was quantifiable in 10/10 boys 1 year prior to pubic hair development measured with LC-MS/MS, and only in 1/10 boys measured with immunoassay. Serum testosterone levels were quantifiable 1.5 years (range 0.5-2.5) earlier using LC-MS/MS.

CONCLUSION: Assessment of longitudinal circulating levels of serum testosterone using a selective LC-MS/MS method proved to be more sensitive in predicting early peripubertal changes in healthy children compared to levels determined by immunoassay.

Original language	English
Journal	Hormone Research in Paediatrics
Volume	82
Issue number	1
Pages (from-to)	12-17
Number of pages	6
ISSN	1663-2818
DOIs	https://doi.org/10.1159/000358560
Publication status	Published - Jul 2014

Keywords

Adolescent
Aging
Child
Female
Follow-Up Studies
Humans
Male
Mass Spectrometry
Puberty
Radioimmunoassay
Sex Characteristics
Testosterone

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Mouritsen, A., Søeborg, T., Johannsen, T. H., Aksglaede, L., Sørensen, K., Hagen, C. P., Mieritz, M. G., Frederiksen, H., Andersson, A-M., & Juul, A. (2014). Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition. Hormone Research in Paediatrics, 82(1), 12-17. https://doi.org/10.1159/000358560

Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition. / Mouritsen, Annette; Søeborg, Tue; Johannsen, Trine Holm et al.

In: Hormone Research in Paediatrics, Vol. 82, No. 1, 07.2014, p. 12-17.

Research output: Contribution to journal › Journal article › Research › peer-review

Mouritsen, A, Søeborg, T, Johannsen, TH, Aksglaede, L, Sørensen, K, Hagen, CP, Mieritz, MG, Frederiksen, H, Andersson, A-M & Juul, A 2014, 'Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition', Hormone Research in Paediatrics, vol. 82, no. 1, pp. 12-17. https://doi.org/10.1159/000358560

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title = "Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition",

abstract = "BACKGROUND: Accurate and selective assessment of testosterone requires use of a sensitive LC-MS/MS method, especially at low levels as those seen in young children.METHODS: The present longitudinal study of 20 healthy children from the Copenhagen Puberty Study followed every 6 months for 5 years evaluates the longitudinal increase in serum testosterone before, during and after pubertal onset quantified by a newly developed LC-MS/MS method in comparison with immunoassay. Testosterone concentrations in serum samples (n = 177) were determined by LC-MS/MS (detection limit 0.1 nmol/l) and by immunoassay (detection limit 0.23 nmol/l).RESULTS: Serum concentrations of testosterone increased gradually with age by both methods. However, serum testosterone was quantifiable in 9/10 girls prior to pubic hair development measured with LC-MS/MS, and in 2/10 girls measured with immunoassay. In boys, testosterone was quantifiable in 10/10 boys 1 year prior to pubic hair development measured with LC-MS/MS, and only in 1/10 boys measured with immunoassay. Serum testosterone levels were quantifiable 1.5 years (range 0.5-2.5) earlier using LC-MS/MS.CONCLUSION: Assessment of longitudinal circulating levels of serum testosterone using a selective LC-MS/MS method proved to be more sensitive in predicting early peripubertal changes in healthy children compared to levels determined by immunoassay.",

keywords = "Adolescent, Aging, Child, Female, Follow-Up Studies, Humans, Male, Mass Spectrometry, Puberty, Radioimmunoassay, Sex Characteristics, Testosterone",

author = "Annette Mouritsen and Tue S{\o}eborg and Johannsen, {Trine Holm} and Lise Aksglaede and Kaspar S{\o}rensen and Hagen, {Casper P} and Mieritz, {Mikkel G} and Hanne Frederiksen and Anna-Maria Andersson and Anders Juul",

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AU - Søeborg, Tue

AU - Johannsen, Trine Holm

AU - Aksglaede, Lise

AU - Sørensen, Kaspar

AU - Hagen, Casper P

AU - Mieritz, Mikkel G

AU - Frederiksen, Hanne

AU - Andersson, Anna-Maria

AU - Juul, Anders

PY - 2014/7

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N2 - BACKGROUND: Accurate and selective assessment of testosterone requires use of a sensitive LC-MS/MS method, especially at low levels as those seen in young children.METHODS: The present longitudinal study of 20 healthy children from the Copenhagen Puberty Study followed every 6 months for 5 years evaluates the longitudinal increase in serum testosterone before, during and after pubertal onset quantified by a newly developed LC-MS/MS method in comparison with immunoassay. Testosterone concentrations in serum samples (n = 177) were determined by LC-MS/MS (detection limit 0.1 nmol/l) and by immunoassay (detection limit 0.23 nmol/l).RESULTS: Serum concentrations of testosterone increased gradually with age by both methods. However, serum testosterone was quantifiable in 9/10 girls prior to pubic hair development measured with LC-MS/MS, and in 2/10 girls measured with immunoassay. In boys, testosterone was quantifiable in 10/10 boys 1 year prior to pubic hair development measured with LC-MS/MS, and only in 1/10 boys measured with immunoassay. Serum testosterone levels were quantifiable 1.5 years (range 0.5-2.5) earlier using LC-MS/MS.CONCLUSION: Assessment of longitudinal circulating levels of serum testosterone using a selective LC-MS/MS method proved to be more sensitive in predicting early peripubertal changes in healthy children compared to levels determined by immunoassay.

AB - BACKGROUND: Accurate and selective assessment of testosterone requires use of a sensitive LC-MS/MS method, especially at low levels as those seen in young children.METHODS: The present longitudinal study of 20 healthy children from the Copenhagen Puberty Study followed every 6 months for 5 years evaluates the longitudinal increase in serum testosterone before, during and after pubertal onset quantified by a newly developed LC-MS/MS method in comparison with immunoassay. Testosterone concentrations in serum samples (n = 177) were determined by LC-MS/MS (detection limit 0.1 nmol/l) and by immunoassay (detection limit 0.23 nmol/l).RESULTS: Serum concentrations of testosterone increased gradually with age by both methods. However, serum testosterone was quantifiable in 9/10 girls prior to pubic hair development measured with LC-MS/MS, and in 2/10 girls measured with immunoassay. In boys, testosterone was quantifiable in 10/10 boys 1 year prior to pubic hair development measured with LC-MS/MS, and only in 1/10 boys measured with immunoassay. Serum testosterone levels were quantifiable 1.5 years (range 0.5-2.5) earlier using LC-MS/MS.CONCLUSION: Assessment of longitudinal circulating levels of serum testosterone using a selective LC-MS/MS method proved to be more sensitive in predicting early peripubertal changes in healthy children compared to levels determined by immunoassay.

KW - Adolescent

KW - Aging

KW - Child

KW - Female

KW - Follow-Up Studies

KW - Humans

KW - Male

KW - Mass Spectrometry

KW - Puberty

KW - Radioimmunoassay

KW - Sex Characteristics

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DO - 10.1159/000358560

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SN - 1663-2818

VL - 82

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JO - Hormone Research in Paediatrics

JF - Hormone Research in Paediatrics

IS - 1

ER -

Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition

Abstract

Keywords

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