Abstract
Cytoplasmic polyadenylation is a post-transcriptional mechanism regulating mRNA stability and translation. The human p53 3'-untranslated region (3'-UTR) contains two regions similar to cytoplasmic polyadenylation elements (CPEs) just upstream of the poly(A) hexanucleotide. Evaluation of the p53 CPE-like elements was performed by luciferase reporter assays, qPCR, and poly(A) assays. Herein, we report the down regulation of a luciferase reporter fused to the p53 3'-UTR, when human CPE-binding protein 1 (hCPEB1) is overexpressed. This inhibition is partially rescued when hCPEB1fused to hGLD-2 [a human cytoplasmic poly(A) polymerase] is overexpressed instead. The stability of a luciferase mRNA containing the p53 3'-UTR downstream, is decreased when hCPEB1 is overexpressed as seen by qPCR. Expression of hGLD-2 restores the mRNA stability. This is due to elongation of the poly(A) tail as seen by a PCR-based poly(A) test and in vitro poly(A) assay. Taken together, our results suggest that hCPEB1 and hGLD-2 are antagonizing factors regulating p53 mRNA stability.
| Original language | English |
|---|---|
| Book series | APMIS Supplementum |
| Volume | 119 |
| Issue number | 11 |
| Pages (from-to) | 769-75 |
| Number of pages | 7 |
| ISSN | 0903-465X |
| DOIs | |
| Publication status | Published - Nov 2011 |
Keywords
- 3' Untranslated Regions
- Cytoplasm
- Humans
- Polyadenylation
- RNA Stability
- RNA, Messenger
- Transcription Factors
- Tumor Suppressor Protein p53
- mRNA Cleavage and Polyadenylation Factors