Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation

H O Hansen; P H Andreasen; S Mandrup; K Kristiansen; J Knudsen

Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation

H O Hansen, P H Andreasen, S Mandrup, K Kristiansen, J Knudsen

Department of Biology

41 Citations (Scopus)

Abstract

The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.

Original language	English
Journal	Biochemical Journal
Volume	277 ( Pt 2)
Pages (from-to)	341-4
Number of pages	3
ISSN	0264-6021
Publication status	Published - 1991

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@article{c3734c500fc911de8478000ea68e967b,

title = "Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation",

abstract = "The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.",

author = "Hansen, {H O} and Andreasen, {P H} and S Mandrup and K Kristiansen and J Knudsen",

note = "Keywords: Acetates; Adipose Tissue; Animals; Carrier Proteins; Cell Differentiation; Cell Line; Cloning, Molecular; Dexamethasone; Diazepam Binding Inhibitor; Gene Library; Kinetics; Lipids; Liver; Mice; RNA, Messenger; Rats; Rats, Inbred Strains; Time Factors",

year = "1991",

language = "English",

volume = "277 ( Pt 2)",

pages = "341--4",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

}

TY - JOUR

T1 - Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation

AU - Hansen, H O

AU - Andreasen, P H

AU - Mandrup, S

AU - Kristiansen, K

AU - Knudsen, J

N1 - Keywords: Acetates; Adipose Tissue; Animals; Carrier Proteins; Cell Differentiation; Cell Line; Cloning, Molecular; Dexamethasone; Diazepam Binding Inhibitor; Gene Library; Kinetics; Lipids; Liver; Mice; RNA, Messenger; Rats; Rats, Inbred Strains; Time Factors

PY - 1991

Y1 - 1991

N2 - The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.

AB - The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.

M3 - Journal article

C2 - 1859362

SN - 0264-6021

VL - 277 ( Pt 2)

SP - 341

EP - 344

JO - Biochemical Journal

JF - Biochemical Journal

ER -

Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation

Abstract

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