In vivo real-time multiphoton imaging of T lymphocytes in the mouse brain after experimental stroke

TY - JOUR

T1 - In vivo real-time multiphoton imaging of T lymphocytes in the mouse brain after experimental stroke

AU - Fumagalli, Stefano

AU - Coles, Jonathan A

AU - Ejlerskov, Patrick

AU - Ortolano, Fabrizio

AU - Bushell, Trevor J

AU - Brewer, James M

AU - De Simoni, Maria-Grazia

AU - Dever, Gary

AU - Garside, Paul

AU - Maffia, Pasquale

AU - Carswell, Hilary V

PY - 2011/5/1

Y1 - 2011/5/1

N2 - BACKGROUND AND PURPOSE-To gain a better understanding of T cell behavior after stroke, we have developed real-time in vivo brain imaging of T cells by multiphoton microscopy after middle cerebral artery occlusion. METHODS-Adult male hCD2-GFP transgenic mice that exhibit green fluorescent protein-labeled T cells underwent permanent left distal middle cerebral artery occlusion by electrocoagulation (n=6) or sham surgery (n=6) and then multiphoton laser imaging 72 hours later. RESULTS-Extravasated T cell number significantly increased after middle cerebral artery occlusion versus sham. Two T cell populations existed after middle cerebral artery occlusion, possibly driven by 2 T cell subpopulations; 1 had significantly lower and the other significantly higher track velocity and displacement rate than sham. CONCLUSIONS-The different motilities and behaviors of T cells observed using our imaging approach after stroke could reveal important mechanisms of immune surveillance for future therapeutic exploitations.

AB - BACKGROUND AND PURPOSE-To gain a better understanding of T cell behavior after stroke, we have developed real-time in vivo brain imaging of T cells by multiphoton microscopy after middle cerebral artery occlusion. METHODS-Adult male hCD2-GFP transgenic mice that exhibit green fluorescent protein-labeled T cells underwent permanent left distal middle cerebral artery occlusion by electrocoagulation (n=6) or sham surgery (n=6) and then multiphoton laser imaging 72 hours later. RESULTS-Extravasated T cell number significantly increased after middle cerebral artery occlusion versus sham. Two T cell populations existed after middle cerebral artery occlusion, possibly driven by 2 T cell subpopulations; 1 had significantly lower and the other significantly higher track velocity and displacement rate than sham. CONCLUSIONS-The different motilities and behaviors of T cells observed using our imaging approach after stroke could reveal important mechanisms of immune surveillance for future therapeutic exploitations.

U2 - 10.1161/STROKEAHA.110.603704

DO - 10.1161/STROKEAHA.110.603704

M3 - Journal article

C2 - 21441145

SN - 0039-2499

VL - 42

SP - 1429

EP - 1436

JO - Stroke

JF - Stroke

IS - 5

ER -