In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs

TY - JOUR

T1 - In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs

AU - Young, J F

AU - Bertram, H C

AU - Theil, P K

AU - Petersen, A-G D

AU - Poulsen, K A

AU - Rasmussen, M

AU - Malmendal, Anders

AU - Nielsen, N C

AU - Vestergaard, M

AU - Oksbjerg, N

PY - 2007/6

Y1 - 2007/6

N2 - Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n=10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. (1)H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes.

AB - Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n=10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. (1)H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes.

U2 - 10.1016/j.meatsci.2006.11.015

DO - 10.1016/j.meatsci.2006.11.015

M3 - Journal article

C2 - 22064305

SN - 0309-1740

VL - 76

SP - 342

EP - 351

JO - Meat Science

JF - Meat Science

IS - 2

ER -