Impaired cutaneous wound healing in mice lacking tetranectin

Kousuke Iba; Naoko Hatakeyama; Takashi Kojima; Masaki Murata; Tadaki Matsumura; Ulla M Wewer; Takuro Wada; Norimasa Sawada; Toshihiko Yamashita

doi:10.1111/j.1524-475X.2008.00447.x

Impaired cutaneous wound healing in mice lacking tetranectin

Kousuke Iba, Naoko Hatakeyama, Takashi Kojima, Masaki Murata, Tadaki Matsumura, Ulla M Wewer, Takuro Wada, Norimasa Sawada, Toshihiko Yamashita

20 Citations (Scopus)

Abstract

Tetranectin was originally purified from human serum on the basis of plasminogen kringle 4-binding properties. Tetranectin enhances plasminogen activation by a tissue-type plasminogen activator so that it has been suggested to play a role in tissue remodeling. We have generated mice with a targeted disruption of the tetranectin gene to elucidate the biological function of tetranectin. In this study, we showed that wound healing was markedly delayed in tetranectin-null mice compared with wild-type mice. A single full-thickness incision was made in the dorsal skin. By 14 days after the incision, the wounds fully healed in all wild-type mice based on the macroscopic closure; in contrast, the progress of wound healing in the tetranectin null mice appeared to be impaired. In histological analysis, wounds of wild-type mice showed complete reepithelialization and healed by 14 days after the incision. However, those of tetranectin-null mice never showed complete reepithelialization at 14 days. At 21 days after the injury, the wound healed and was covered with an epidermis. These results supported the fact that tetranectin may play a role in the wound healing process.

Original language	English
Journal	Wound Repair and Regeneration
Volume	17
Issue number	1
Pages (from-to)	108-12
Number of pages	4
ISSN	1067-1927
DOIs	https://doi.org/10.1111/j.1524-475X.2008.00447.x
Publication status	Published - 2009

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title = "Impaired cutaneous wound healing in mice lacking tetranectin",

abstract = "Tetranectin was originally purified from human serum on the basis of plasminogen kringle 4-binding properties. Tetranectin enhances plasminogen activation by a tissue-type plasminogen activator so that it has been suggested to play a role in tissue remodeling. We have generated mice with a targeted disruption of the tetranectin gene to elucidate the biological function of tetranectin. In this study, we showed that wound healing was markedly delayed in tetranectin-null mice compared with wild-type mice. A single full-thickness incision was made in the dorsal skin. By 14 days after the incision, the wounds fully healed in all wild-type mice based on the macroscopic closure; in contrast, the progress of wound healing in the tetranectin null mice appeared to be impaired. In histological analysis, wounds of wild-type mice showed complete reepithelialization and healed by 14 days after the incision. However, those of tetranectin-null mice never showed complete reepithelialization at 14 days. At 21 days after the injury, the wound healed and was covered with an epidermis. These results supported the fact that tetranectin may play a role in the wound healing process.",

author = "Kousuke Iba and Naoko Hatakeyama and Takashi Kojima and Masaki Murata and Tadaki Matsumura and Wewer, {Ulla M} and Takuro Wada and Norimasa Sawada and Toshihiko Yamashita",

note = "Keywords: Animals; Lectins, C-Type; Male; Mice; Models, Animal; Reverse Transcriptase Polymerase Chain Reaction; Skin; Wound Healing",

year = "2009",

doi = "10.1111/j.1524-475X.2008.00447.x",

language = "English",

volume = "17",

pages = "108--12",

journal = "Wound Repair and Regeneration",

issn = "1067-1927",

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number = "1",

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TY - JOUR

T1 - Impaired cutaneous wound healing in mice lacking tetranectin

AU - Iba, Kousuke

AU - Hatakeyama, Naoko

AU - Kojima, Takashi

AU - Murata, Masaki

AU - Matsumura, Tadaki

AU - Wewer, Ulla M

AU - Wada, Takuro

AU - Sawada, Norimasa

AU - Yamashita, Toshihiko

N1 - Keywords: Animals; Lectins, C-Type; Male; Mice; Models, Animal; Reverse Transcriptase Polymerase Chain Reaction; Skin; Wound Healing

PY - 2009

Y1 - 2009

N2 - Tetranectin was originally purified from human serum on the basis of plasminogen kringle 4-binding properties. Tetranectin enhances plasminogen activation by a tissue-type plasminogen activator so that it has been suggested to play a role in tissue remodeling. We have generated mice with a targeted disruption of the tetranectin gene to elucidate the biological function of tetranectin. In this study, we showed that wound healing was markedly delayed in tetranectin-null mice compared with wild-type mice. A single full-thickness incision was made in the dorsal skin. By 14 days after the incision, the wounds fully healed in all wild-type mice based on the macroscopic closure; in contrast, the progress of wound healing in the tetranectin null mice appeared to be impaired. In histological analysis, wounds of wild-type mice showed complete reepithelialization and healed by 14 days after the incision. However, those of tetranectin-null mice never showed complete reepithelialization at 14 days. At 21 days after the injury, the wound healed and was covered with an epidermis. These results supported the fact that tetranectin may play a role in the wound healing process.

AB - Tetranectin was originally purified from human serum on the basis of plasminogen kringle 4-binding properties. Tetranectin enhances plasminogen activation by a tissue-type plasminogen activator so that it has been suggested to play a role in tissue remodeling. We have generated mice with a targeted disruption of the tetranectin gene to elucidate the biological function of tetranectin. In this study, we showed that wound healing was markedly delayed in tetranectin-null mice compared with wild-type mice. A single full-thickness incision was made in the dorsal skin. By 14 days after the incision, the wounds fully healed in all wild-type mice based on the macroscopic closure; in contrast, the progress of wound healing in the tetranectin null mice appeared to be impaired. In histological analysis, wounds of wild-type mice showed complete reepithelialization and healed by 14 days after the incision. However, those of tetranectin-null mice never showed complete reepithelialization at 14 days. At 21 days after the injury, the wound healed and was covered with an epidermis. These results supported the fact that tetranectin may play a role in the wound healing process.

U2 - 10.1111/j.1524-475X.2008.00447.x

DO - 10.1111/j.1524-475X.2008.00447.x

M3 - Journal article

C2 - 19152657

SN - 1067-1927

VL - 17

SP - 108

EP - 112

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

IS - 1

ER -

Impaired cutaneous wound healing in mice lacking tetranectin

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