Impact of BCRP/MXR, MRP1 and MDR1/P-Glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer cells

Ulrike Stein; Hermann Lage; Andreas Jordan; Wolfgang Walther; Susan E Bates; Thomas Litman; Peter Hohenberger; Manfred Dietel

Impact of BCRP/MXR, MRP1 and MDR1/P-Glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer cells

Ulrike Stein, Hermann Lage, Andreas Jordan, Wolfgang Walther, Susan E Bates, Thomas Litman, Peter Hohenberger, Manfred Dietel

Cell Biology and Physiology

33 Citations (Scopus)

Abstract

The impact of the ABC transporters breast cancer resistance protein/mitoxantrone resistance associated transporter (BCRP/MXR), multidrug resistance-associated protein 1 (MRP1) and multidrug resistance gene-1/P-glycoprotein (MDR1/PGP) on the multidrug resistance (MDR) phenotype in chemoresistance and thermoresistance was investigated in the parental human gastric carcinoma cell line EPG85-257P, the atypical MDR subline EPG85-257RNOV, the classical MDR subline EPG85-257RDB and their thermoresistant counterparts EPG85-257P-TR, EPG85-257RNOV-TR and EPG85-257RDB-TR. Within the atypical MDR subline EPG85-257RNOV expression of BCRP/MXR and of MRP1 were clearly enhanced (vs. parental and classical MDR lines). MDR1/PGP expression was distinctly elevated in the classical MDR subline EPG85-257RDB (vs. parental and atypical MDR sublines). In all thermoresistant counterparts basal expression of BCRP/MXR, MRP1 and MDR1/PGP was increased relative to thermosensitive sublines. Although it could be shown that the overexpressed ABC transporters were functionally active, however, no decreased drug accumulations of doxorubicin, mitoxantrone and rhodamine 123 were observed. Thus, expression of BCRP/MXR, MRP1 and MDR1/PGP was found to be dependent on the appropriate type of chemoresistance; correlating with a classical or atypical MDR phenotype. Within the thermoresistant variants, however, the increase in ABC transporter expression did obviously not influence the MDR phenotype.

Original language	English
Journal	International journal of cancer. Journal international du cancer
Volume	97
Issue number	6
Pages (from-to)	751-60
Number of pages	10
ISSN	0020-7136
Publication status	Published - 20 Feb 2002

Keywords

ATP-Binding Cassette Transporters
Antibiotics, Antineoplastic
Antineoplastic Agents
Calcium Channel Blockers
DNA Primers
DNA-Binding Proteins
Doxorubicin
Drug Resistance, Multiple
Fluorescent Dyes
Gastrointestinal Neoplasms
Gene Expression Regulation
Genes, MDR
Hot Temperature
Humans
Indenes
Mitoxantrone
Multidrug Resistance-Associated Proteins
Mycotoxins
Neoplasm Proteins
P-Glycoprotein
Propionates
Quinolines
RNA, Messenger
RNA, Neoplasm
Reverse Transcriptase Polymerase Chain Reaction
Rhodamine 123
Stomach Neoplasms
Verapamil

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Impact of BCRP/MXR, MRP1 and MDR1/P-Glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer cells. / Stein, Ulrike; Lage, Hermann; Jordan, Andreas et al.

In: International journal of cancer. Journal international du cancer, Vol. 97, No. 6, 20.02.2002, p. 751-60.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Impact of BCRP/MXR, MRP1 and MDR1/P-Glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer cells",

abstract = "The impact of the ABC transporters breast cancer resistance protein/mitoxantrone resistance associated transporter (BCRP/MXR), multidrug resistance-associated protein 1 (MRP1) and multidrug resistance gene-1/P-glycoprotein (MDR1/PGP) on the multidrug resistance (MDR) phenotype in chemoresistance and thermoresistance was investigated in the parental human gastric carcinoma cell line EPG85-257P, the atypical MDR subline EPG85-257RNOV, the classical MDR subline EPG85-257RDB and their thermoresistant counterparts EPG85-257P-TR, EPG85-257RNOV-TR and EPG85-257RDB-TR. Within the atypical MDR subline EPG85-257RNOV expression of BCRP/MXR and of MRP1 were clearly enhanced (vs. parental and classical MDR lines). MDR1/PGP expression was distinctly elevated in the classical MDR subline EPG85-257RDB (vs. parental and atypical MDR sublines). In all thermoresistant counterparts basal expression of BCRP/MXR, MRP1 and MDR1/PGP was increased relative to thermosensitive sublines. Although it could be shown that the overexpressed ABC transporters were functionally active, however, no decreased drug accumulations of doxorubicin, mitoxantrone and rhodamine 123 were observed. Thus, expression of BCRP/MXR, MRP1 and MDR1/PGP was found to be dependent on the appropriate type of chemoresistance; correlating with a classical or atypical MDR phenotype. Within the thermoresistant variants, however, the increase in ABC transporter expression did obviously not influence the MDR phenotype.",

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author = "Ulrike Stein and Hermann Lage and Andreas Jordan and Wolfgang Walther and Bates, {Susan E} and Thomas Litman and Peter Hohenberger and Manfred Dietel",

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language = "English",

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journal = "Radiation Oncology Investigations",

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TY - JOUR

T1 - Impact of BCRP/MXR, MRP1 and MDR1/P-Glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer cells

AU - Stein, Ulrike

AU - Lage, Hermann

AU - Jordan, Andreas

AU - Walther, Wolfgang

AU - Bates, Susan E

AU - Litman, Thomas

AU - Hohenberger, Peter

AU - Dietel, Manfred

PY - 2002/2/20

Y1 - 2002/2/20

N2 - The impact of the ABC transporters breast cancer resistance protein/mitoxantrone resistance associated transporter (BCRP/MXR), multidrug resistance-associated protein 1 (MRP1) and multidrug resistance gene-1/P-glycoprotein (MDR1/PGP) on the multidrug resistance (MDR) phenotype in chemoresistance and thermoresistance was investigated in the parental human gastric carcinoma cell line EPG85-257P, the atypical MDR subline EPG85-257RNOV, the classical MDR subline EPG85-257RDB and their thermoresistant counterparts EPG85-257P-TR, EPG85-257RNOV-TR and EPG85-257RDB-TR. Within the atypical MDR subline EPG85-257RNOV expression of BCRP/MXR and of MRP1 were clearly enhanced (vs. parental and classical MDR lines). MDR1/PGP expression was distinctly elevated in the classical MDR subline EPG85-257RDB (vs. parental and atypical MDR sublines). In all thermoresistant counterparts basal expression of BCRP/MXR, MRP1 and MDR1/PGP was increased relative to thermosensitive sublines. Although it could be shown that the overexpressed ABC transporters were functionally active, however, no decreased drug accumulations of doxorubicin, mitoxantrone and rhodamine 123 were observed. Thus, expression of BCRP/MXR, MRP1 and MDR1/PGP was found to be dependent on the appropriate type of chemoresistance; correlating with a classical or atypical MDR phenotype. Within the thermoresistant variants, however, the increase in ABC transporter expression did obviously not influence the MDR phenotype.

AB - The impact of the ABC transporters breast cancer resistance protein/mitoxantrone resistance associated transporter (BCRP/MXR), multidrug resistance-associated protein 1 (MRP1) and multidrug resistance gene-1/P-glycoprotein (MDR1/PGP) on the multidrug resistance (MDR) phenotype in chemoresistance and thermoresistance was investigated in the parental human gastric carcinoma cell line EPG85-257P, the atypical MDR subline EPG85-257RNOV, the classical MDR subline EPG85-257RDB and their thermoresistant counterparts EPG85-257P-TR, EPG85-257RNOV-TR and EPG85-257RDB-TR. Within the atypical MDR subline EPG85-257RNOV expression of BCRP/MXR and of MRP1 were clearly enhanced (vs. parental and classical MDR lines). MDR1/PGP expression was distinctly elevated in the classical MDR subline EPG85-257RDB (vs. parental and atypical MDR sublines). In all thermoresistant counterparts basal expression of BCRP/MXR, MRP1 and MDR1/PGP was increased relative to thermosensitive sublines. Although it could be shown that the overexpressed ABC transporters were functionally active, however, no decreased drug accumulations of doxorubicin, mitoxantrone and rhodamine 123 were observed. Thus, expression of BCRP/MXR, MRP1 and MDR1/PGP was found to be dependent on the appropriate type of chemoresistance; correlating with a classical or atypical MDR phenotype. Within the thermoresistant variants, however, the increase in ABC transporter expression did obviously not influence the MDR phenotype.

KW - ATP-Binding Cassette Transporters

KW - Antibiotics, Antineoplastic

KW - Antineoplastic Agents

KW - Calcium Channel Blockers

KW - DNA Primers

KW - DNA-Binding Proteins

KW - Doxorubicin

KW - Drug Resistance, Multiple

KW - Fluorescent Dyes

KW - Gastrointestinal Neoplasms

KW - Gene Expression Regulation

KW - Genes, MDR

KW - Hot Temperature

KW - Humans

KW - Indenes

KW - Mitoxantrone

KW - Multidrug Resistance-Associated Proteins

KW - Mycotoxins

KW - Neoplasm Proteins

KW - P-Glycoprotein

KW - Propionates

KW - Quinolines

KW - RNA, Messenger

KW - RNA, Neoplasm

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Rhodamine 123

KW - Stomach Neoplasms

KW - Verapamil

M3 - Journal article

C2 - 11857350

SN - 0020-7136

VL - 97

SP - 751

EP - 760

JO - Radiation Oncology Investigations

JF - Radiation Oncology Investigations

IS - 6

ER -

Impact of BCRP/MXR, MRP1 and MDR1/P-Glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer cells

Abstract

Keywords

UN SDGs

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