Fluorescence spectroscopy and multi-way techniques. PARAFAC

TY - JOUR

T1 - Fluorescence spectroscopy and multi-way techniques. PARAFAC

AU - Murphy, Kathleen R.

AU - Stedmon, Colin

AU - Graeber, Daniel

AU - Bro, Rasmus

PY - 2013/12/7

Y1 - 2013/12/7

N2 - PARAllel FACtor analysis (PARAFAC) is increasingly used to decompose fluorescence excitation emission matrices (EEMs) into their underlying chemical components. In the ideal case where fluorescence conforms to Beers Law, this process can lead to the mathematical identification and quantification of independently varying fluorophores. However, many practical and analytical hurdles stand between EEM datasets and their chemical interpretation. This article provides a tutorial in the practical application of PARAFAC to fluorescence datasets, demonstrated using a dissolved organic matter (DOM) fluorescence dataset. A new toolbox for MATLAB is presented to support improved visualisation and sensitivity analyses of PARAFAC models in fluorescence spectroscopy.

AB - PARAllel FACtor analysis (PARAFAC) is increasingly used to decompose fluorescence excitation emission matrices (EEMs) into their underlying chemical components. In the ideal case where fluorescence conforms to Beers Law, this process can lead to the mathematical identification and quantification of independently varying fluorophores. However, many practical and analytical hurdles stand between EEM datasets and their chemical interpretation. This article provides a tutorial in the practical application of PARAFAC to fluorescence datasets, demonstrated using a dissolved organic matter (DOM) fluorescence dataset. A new toolbox for MATLAB is presented to support improved visualisation and sensitivity analyses of PARAFAC models in fluorescence spectroscopy.

U2 - 10.1039/c3ay41160e

DO - 10.1039/c3ay41160e

M3 - Review

SN - 1759-9660

VL - 5

SP - 6557

EP - 6566

JO - Analytical Methods

JF - Analytical Methods

IS - 23

ER -