Fast, cross cultivar determination of total carotenoids in intact carrot, tissue by Raman spectroscopy and Partial Least Squares calibration

Anders Juul Lawaetz; Signe M. U. Christensen; Sabine Karin Clausen; Bjarne Jørnsgård; Søren Kjærsgaard Rasmussen; Sven Bode Andersen; Åsmund Rinnan

doi:10.1016/j.foodchem.2016.02.107

Fast, cross cultivar determination of total carotenoids in intact carrot, tissue by Raman spectroscopy and Partial Least Squares calibration

Anders Juul Lawaetz, Signe M. U. Christensen, Sabine Karin Clausen, Bjarne Jørnsgård, Søren Kjærsgaard Rasmussen, Sven Bode Andersen, Åsmund Rinnan

14 Citations (Scopus)

Abstract

In order to speed up the breeding of orange carrots for high carotenoid content it is imperative to develop a fast and non-destructive technique. 332 roots from 86 carrot varieties grown in 2014 at the experimental farm in Høje Taastrup (DK) form the basis of this study. All roots were measured by Raman spectroscopy. The carotenoid content of the very same roots was estimated through a wet chemistry method coupled with UV-VIS at 447 nm and 540 nm. For the Raman spectroscopy, measurements were made on a cross section disk approximately 10 cm from the root top at three different positions in the phloem. Since the top of the carrot is intact, it may still be used for growing. The final calibration model shows an uncertainty (RMSECV) of 20.5 ppm, and a R² = 0.86. It has thus proven to be well suited for prediction of carotenoids in orange carrots, and especially for ranking them according to the content.

Original language	English
Journal	Food Chemistry
Volume	204
Pages (from-to)	7-13
Number of pages	7
ISSN	0308-8146
DOIs	https://doi.org/10.1016/j.foodchem.2016.02.107
Publication status	Published - 1 Aug 2016

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title = "Fast, cross cultivar determination of total carotenoids in intact carrot, tissue by Raman spectroscopy and Partial Least Squares calibration",

abstract = "In order to speed up the breeding of orange carrots for high carotenoid content it is imperative to develop a fast and non-destructive technique. 332 roots from 86 carrot varieties grown in 2014 at the experimental farm in H{\o}je Taastrup (DK) form the basis of this study. All roots were measured by Raman spectroscopy. The carotenoid content of the very same roots was estimated through a wet chemistry method coupled with UV-VIS at 447 nm and 540 nm. For the Raman spectroscopy, measurements were made on a cross section disk approximately 10 cm from the root top at three different positions in the phloem. Since the top of the carrot is intact, it may still be used for growing. The final calibration model shows an uncertainty (RMSECV) of 20.5 ppm, and a R2 = 0.86. It has thus proven to be well suited for prediction of carotenoids in orange carrots, and especially for ranking them according to the content.",

author = "Lawaetz, {Anders Juul} and Christensen, {Signe M. U.} and Clausen, {Sabine Karin} and Bjarne J{\o}rnsg{\aa}rd and Rasmussen, {S{\o}ren Kj{\ae}rsgaard} and Andersen, {Sven Bode} and {\AA}smund Rinnan",

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doi = "10.1016/j.foodchem.2016.02.107",

language = "English",

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T1 - Fast, cross cultivar determination of total carotenoids in intact carrot, tissue by Raman spectroscopy and Partial Least Squares calibration

AU - Lawaetz, Anders Juul

AU - Christensen, Signe M. U.

AU - Clausen, Sabine Karin

AU - Jørnsgård, Bjarne

AU - Rasmussen, Søren Kjærsgaard

AU - Andersen, Sven Bode

AU - Rinnan, Åsmund

N1 - Administrerende institut: Department of Food Science

PY - 2016/8/1

Y1 - 2016/8/1

N2 - In order to speed up the breeding of orange carrots for high carotenoid content it is imperative to develop a fast and non-destructive technique. 332 roots from 86 carrot varieties grown in 2014 at the experimental farm in Høje Taastrup (DK) form the basis of this study. All roots were measured by Raman spectroscopy. The carotenoid content of the very same roots was estimated through a wet chemistry method coupled with UV-VIS at 447 nm and 540 nm. For the Raman spectroscopy, measurements were made on a cross section disk approximately 10 cm from the root top at three different positions in the phloem. Since the top of the carrot is intact, it may still be used for growing. The final calibration model shows an uncertainty (RMSECV) of 20.5 ppm, and a R2 = 0.86. It has thus proven to be well suited for prediction of carotenoids in orange carrots, and especially for ranking them according to the content.

AB - In order to speed up the breeding of orange carrots for high carotenoid content it is imperative to develop a fast and non-destructive technique. 332 roots from 86 carrot varieties grown in 2014 at the experimental farm in Høje Taastrup (DK) form the basis of this study. All roots were measured by Raman spectroscopy. The carotenoid content of the very same roots was estimated through a wet chemistry method coupled with UV-VIS at 447 nm and 540 nm. For the Raman spectroscopy, measurements were made on a cross section disk approximately 10 cm from the root top at three different positions in the phloem. Since the top of the carrot is intact, it may still be used for growing. The final calibration model shows an uncertainty (RMSECV) of 20.5 ppm, and a R2 = 0.86. It has thus proven to be well suited for prediction of carotenoids in orange carrots, and especially for ranking them according to the content.

U2 - 10.1016/j.foodchem.2016.02.107

DO - 10.1016/j.foodchem.2016.02.107

M3 - Journal article

C2 - 26988469

SN - 0308-8146

VL - 204

SP - 7

EP - 13

JO - Food Chemistry

JF - Food Chemistry

ER -

Fast, cross cultivar determination of total carotenoids in intact carrot, tissue by Raman spectroscopy and Partial Least Squares calibration

Abstract

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