Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus

Jinlong Qiu; Berthe Katrine Fiil; Klaus Petersen; Henrik Bjørn Nielsen; Christopher J Botanga; Stephan Pascal Thorgrimsen; Kristoffer Palma; Maria Cristina Suarez Rodriguez; Signe Sandbech-Clausen; Jacek Lichota; Peter Brodersen; Klaus D Grasser; Ole Mattsson; Jane Glazebrook; John Mundy; Morten Petersen

doi:10.1038/emboj.2008.147

Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus

Jinlong Qiu, Berthe Katrine Fiil, Klaus Petersen, Henrik Bjørn Nielsen, Christopher J Botanga, Stephan Pascal Thorgrimsen, Kristoffer Palma, Maria Cristina Suarez Rodriguez, Signe Sandbech-Clausen, Jacek Lichota, Peter Brodersen, Klaus D Grasser, Ole Mattsson, Jane Glazebrook, John Mundy, Morten Petersen

314 Citations (Scopus)

Abstract

Plant and animal perception of microbes through pathogen surveillance proteins leads to MAP kinase signalling and the expression of defence genes. However, little is known about how plant MAP kinases regulate specific gene expression. We report that, in the absence of pathogens, Arabidopsis MAP kinase 4 (MPK4) exists in nuclear complexes with the WRKY33 transcription factor. This complex depends on the MPK4 substrate MKS1. Challenge with Pseudomonas syringae or flagellin leads to the activation of MPK4 and phosphorylation of MKS1. Subsequently, complexes with MKS1 and WRKY33 are released from MPK4, and WRKY33 targets the promoter of PHYTOALEXIN DEFICIENT3 (PAD3) encoding an enzyme required for the synthesis of antimicrobial camalexin. Hence, wrky33 mutants are impaired in the accumulation of PAD3 mRNA and camalexin production upon infection. That WRKY33 is an effector of MPK4 is further supported by the suppression of PAD3 expression in mpk4-wrky33 double mutant backgrounds. Our data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation.

Original language	English
Journal	EMBO Journal
Volume	27
Issue number	16
Pages (from-to)	2214-2221
Number of pages	8
ISSN	0261-4189
DOIs	https://doi.org/10.1038/emboj.2008.147
Publication status	Published - 2008

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Qiu, J., Fiil, B. K., Petersen, K., Nielsen, H. B., Botanga, C. J., Thorgrimsen, S. P., Palma, K., Suarez Rodriguez, M. C., Sandbech-Clausen, S., Lichota, J., Brodersen, P., Grasser, K. D., Mattsson, O., Glazebrook, J., Mundy, J., & Petersen, M. (2008). Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus. EMBO Journal, 27(16), 2214-2221. https://doi.org/10.1038/emboj.2008.147

Qiu, J, Fiil, BK, Petersen, K, Nielsen, HB, Botanga, CJ, Thorgrimsen, SP, Palma, K, Suarez Rodriguez, MC, Sandbech-Clausen, S, Lichota, J, Brodersen, P, Grasser, KD, Mattsson, O, Glazebrook, J, Mundy, J & Petersen, M 2008, 'Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus', EMBO Journal, vol. 27, no. 16, pp. 2214-2221. https://doi.org/10.1038/emboj.2008.147

@article{17aa93d0a51711df928f000ea68e967b,

title = "Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus",

abstract = "Plant and animal perception of microbes through pathogen surveillance proteins leads to MAP kinase signalling and the expression of defence genes. However, little is known about how plant MAP kinases regulate specific gene expression. We report that, in the absence of pathogens, Arabidopsis MAP kinase 4 (MPK4) exists in nuclear complexes with the WRKY33 transcription factor. This complex depends on the MPK4 substrate MKS1. Challenge with Pseudomonas syringae or flagellin leads to the activation of MPK4 and phosphorylation of MKS1. Subsequently, complexes with MKS1 and WRKY33 are released from MPK4, and WRKY33 targets the promoter of PHYTOALEXIN DEFICIENT3 (PAD3) encoding an enzyme required for the synthesis of antimicrobial camalexin. Hence, wrky33 mutants are impaired in the accumulation of PAD3 mRNA and camalexin production upon infection. That WRKY33 is an effector of MPK4 is further supported by the suppression of PAD3 expression in mpk4-wrky33 double mutant backgrounds. Our data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation.",

author = "Jinlong Qiu and Fiil, {Berthe Katrine} and Klaus Petersen and Nielsen, {Henrik Bj{\o}rn} and Botanga, {Christopher J} and Thorgrimsen, {Stephan Pascal} and Kristoffer Palma and {Suarez Rodriguez}, {Maria Cristina} and Signe Sandbech-Clausen and Jacek Lichota and Peter Brodersen and Grasser, {Klaus D} and Ole Mattsson and Jane Glazebrook and John Mundy and Morten Petersen",

note = "Keywords:Arabidopsis, defence response, MAP kinase, target genes, transcription factor",

year = "2008",

doi = "10.1038/emboj.2008.147",

language = "English",

volume = "27",

pages = "2214--2221",

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T1 - Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus

AU - Qiu, Jinlong

AU - Fiil, Berthe Katrine

AU - Petersen, Klaus

AU - Nielsen, Henrik Bjørn

AU - Botanga, Christopher J

AU - Thorgrimsen, Stephan Pascal

AU - Palma, Kristoffer

AU - Suarez Rodriguez, Maria Cristina

AU - Sandbech-Clausen, Signe

AU - Lichota, Jacek

AU - Brodersen, Peter

AU - Grasser, Klaus D

AU - Mattsson, Ole

AU - Glazebrook, Jane

AU - Mundy, John

AU - Petersen, Morten

N1 - Keywords:Arabidopsis, defence response, MAP kinase, target genes, transcription factor

PY - 2008

Y1 - 2008

N2 - Plant and animal perception of microbes through pathogen surveillance proteins leads to MAP kinase signalling and the expression of defence genes. However, little is known about how plant MAP kinases regulate specific gene expression. We report that, in the absence of pathogens, Arabidopsis MAP kinase 4 (MPK4) exists in nuclear complexes with the WRKY33 transcription factor. This complex depends on the MPK4 substrate MKS1. Challenge with Pseudomonas syringae or flagellin leads to the activation of MPK4 and phosphorylation of MKS1. Subsequently, complexes with MKS1 and WRKY33 are released from MPK4, and WRKY33 targets the promoter of PHYTOALEXIN DEFICIENT3 (PAD3) encoding an enzyme required for the synthesis of antimicrobial camalexin. Hence, wrky33 mutants are impaired in the accumulation of PAD3 mRNA and camalexin production upon infection. That WRKY33 is an effector of MPK4 is further supported by the suppression of PAD3 expression in mpk4-wrky33 double mutant backgrounds. Our data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation.

AB - Plant and animal perception of microbes through pathogen surveillance proteins leads to MAP kinase signalling and the expression of defence genes. However, little is known about how plant MAP kinases regulate specific gene expression. We report that, in the absence of pathogens, Arabidopsis MAP kinase 4 (MPK4) exists in nuclear complexes with the WRKY33 transcription factor. This complex depends on the MPK4 substrate MKS1. Challenge with Pseudomonas syringae or flagellin leads to the activation of MPK4 and phosphorylation of MKS1. Subsequently, complexes with MKS1 and WRKY33 are released from MPK4, and WRKY33 targets the promoter of PHYTOALEXIN DEFICIENT3 (PAD3) encoding an enzyme required for the synthesis of antimicrobial camalexin. Hence, wrky33 mutants are impaired in the accumulation of PAD3 mRNA and camalexin production upon infection. That WRKY33 is an effector of MPK4 is further supported by the suppression of PAD3 expression in mpk4-wrky33 double mutant backgrounds. Our data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation.

U2 - 10.1038/emboj.2008.147

DO - 10.1038/emboj.2008.147

M3 - Journal article

C2 - 18650934

SN - 0261-4189

VL - 27

SP - 2214

EP - 2221

JO - E M B O Journal

JF - E M B O Journal

IS - 16

ER -

Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus

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