Effect of tamoxifen on the receptor-positive T61 and the receptor-negative T60 human breast carcinomas grown in nude mice

TY - JOUR

T1 - Effect of tamoxifen on the receptor-positive T61 and the receptor-negative T60 human breast carcinomas grown in nude mice

AU - Brünner, N

AU - Spang-Thomsen, M

AU - Vindeløv, L

AU - Wolff, J

AU - Engelholm, S A

N1 - Keywords: Animals; Breast Neoplasms; Cell Count; Cell Cycle; DNA, Neoplasm; Dose-Response Relationship, Drug; Female; Flow Cytometry; Humans; Male; Mice; Mice, Nude; Neoplasm Transplantation; Receptors, Estrogen; Receptors, Progesterone; Tamoxifen; Time Factors

PY - 1985

Y1 - 1985

N2 - A study was made of the in vivo effect of the anti-oestrogen tamoxifen on the growth and cell cycle kinetics of the oestrogen and progesterone receptor-positive T61 human breast carcinoma and of the oestrogen and progesterone receptor-negative T60 human breast carcinoma grown in nude mice. The T61 tumour was exposed to single doses of 0.1, 1.0, 5 or 10 mg tamoxifen, resulting in serum concentrations 1 day after treatment ranging from less than 27 to 134 nM. The T60 tumour was exposed to a single dose of 1.0 mg tamoxifen. The effect on the tumour growth curves was determined according to a transformed Gompertz function, and the effect on the cell cycle distributions was estimated by flow cytometric DNA analysis on tumour tissue obtained by fine-needle aspirations at intervals after the treatment. The results showed that in the T61 tumour, tamoxifen induced a dose-related growth inhibition, whereas it had no concomitant effect on the cell cycle distribution of the tumour. The treatment had no effect on the T60 tumour. The results indicate that tamoxifen is unsuitable as a synchronizing agent in human breast tumours with responses to the drug similar to those of the T61 and T60 breast tumours.

AB - A study was made of the in vivo effect of the anti-oestrogen tamoxifen on the growth and cell cycle kinetics of the oestrogen and progesterone receptor-positive T61 human breast carcinoma and of the oestrogen and progesterone receptor-negative T60 human breast carcinoma grown in nude mice. The T61 tumour was exposed to single doses of 0.1, 1.0, 5 or 10 mg tamoxifen, resulting in serum concentrations 1 day after treatment ranging from less than 27 to 134 nM. The T60 tumour was exposed to a single dose of 1.0 mg tamoxifen. The effect on the tumour growth curves was determined according to a transformed Gompertz function, and the effect on the cell cycle distributions was estimated by flow cytometric DNA analysis on tumour tissue obtained by fine-needle aspirations at intervals after the treatment. The results showed that in the T61 tumour, tamoxifen induced a dose-related growth inhibition, whereas it had no concomitant effect on the cell cycle distribution of the tumour. The treatment had no effect on the T60 tumour. The results indicate that tamoxifen is unsuitable as a synchronizing agent in human breast tumours with responses to the drug similar to those of the T61 and T60 breast tumours.

M3 - Journal article

C2 - 4076295

SN - 0277-5379

VL - 21

SP - 1349

EP - 1354

JO - European Journal of Cancer and Clinical Oncology

JF - European Journal of Cancer and Clinical Oncology

IS - 11

ER -