Effect of melphalan on growth curves and cell cycle distribution of four human small cell carcinomas of the lung grown in nude mice

TY - JOUR

T1 - Effect of melphalan on growth curves and cell cycle distribution of four human small cell carcinomas of the lung grown in nude mice

AU - Engelholm, S A

AU - Spang-Thomsen, M

AU - Vindeløv, L L

AU - Brünner, N

N1 - Keywords: Animals; Carcinoma, Small Cell; Cell Cycle; Cell Line; DNA; Dose-Response Relationship, Drug; Drug Resistance; Flow Cytometry; Growth; Humans; Lung Neoplasms; Melphalan; Mice; Mice, Nude; Neoplasm Transplantation

PY - 1986

Y1 - 1986

N2 - Four human small cell carcinomas of the lung grown in nude mice were exposed to melphalan. Two of the tumors were derived from subpopulations isolated by in vitro cloning from the same tumor biopsy. The chemosensitivity of the tumors was determined by calculating the specific growth delay. Drug-induced changes in the cell cycle were detected by flow cytometric DNA analysis. The specific growth delay of the tumors was very different with the greatest differences between the two subpopulations originating from the same tumor. Melphalan induced a dose-related S phase accumulation in three sensitive tumors, whereas no changes were seen in a resistant tumor. Furthermore, the amount of S phase accumulation reflected the sensitivity to melphalan. The results suggest that heterogeneity in chemosensitivity is an important reason for chemotherapy failures.

AB - Four human small cell carcinomas of the lung grown in nude mice were exposed to melphalan. Two of the tumors were derived from subpopulations isolated by in vitro cloning from the same tumor biopsy. The chemosensitivity of the tumors was determined by calculating the specific growth delay. Drug-induced changes in the cell cycle were detected by flow cytometric DNA analysis. The specific growth delay of the tumors was very different with the greatest differences between the two subpopulations originating from the same tumor. Melphalan induced a dose-related S phase accumulation in three sensitive tumors, whereas no changes were seen in a resistant tumor. Furthermore, the amount of S phase accumulation reflected the sensitivity to melphalan. The results suggest that heterogeneity in chemosensitivity is an important reason for chemotherapy failures.

M3 - Journal article

C2 - 3017779

SN - 0304-3568

VL - 54

SP - 138

EP - 148

JO - Experimental Cell Biology

JF - Experimental Cell Biology

IS - 3

ER -