Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides

Cornelis A. Van Walree; Yumiko Sakuragi; Dorte B. Steensgaard; Carola S. Bösinger; Niels-Ulrik Frigaard; Raymond P. Cox; Alfred R. Holzwart; Mette Miller

doi:10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides

Cornelis A. Van Walree, Yumiko Sakuragi, Dorte B. Steensgaard, Carola S. Bösinger, Niels-Ulrik Frigaard, Raymond P. Cox, Alfred R. Holzwart, Mette Miller^*

^*Corresponding author for this work

24 Citations (Scopus)

Abstract

Chlorosomes isolated from two types of green sulfur bacteria, Chlorobium tepidum which contains bacteriochlorophyll c (BChl c) and the BChl e-containing Chlorobium phaeobacteroides, were subjected to alkaline treatment (pH 12.7 at 40°C for 20 min). This caused selective degradation of BChl a, whereas BChl c or e were not affected. Chlorobiumquinone in the chlorosomes was partially degraded by the alkaline treatment but menaquinone was unchanged. Fluorescence decay kinetics showed that alkaline treatment disrupted energy transfer from BChl c or e to BChl a under reducing conditions. However, this did not give rise to any substantial increase in the excited state lifetime of BChl e in C. phaeobacteroides chlorosomes, while for C. tepidum a decrease in the BChl c lifetime was found. The steady-state fluorescence of chlorosomes is highly dependent on the redox potential such that emission is quenched in oxidizing environments. Alkaline treatment diminished this quenching effect and caused a doubling in the BChl c or e emission intensity under aerobic conditions. Single-photon timing experiments confirmed that alkaline treatment inhibits the energy trapping process operative under aerobic conditions. These effects of alkaline treatment on the fluorescence intensity and decay kinetics are likely to be related to the depletion in BChI a or in Chlorobiumquinone or a combination of these.

Original language	English
Journal	Photochemistry and Photobiology
Volume	69
Issue number	3
Pages (from-to)	322-328
Number of pages	7
ISSN	0031-8655
DOIs	https://doi.org/10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2
Publication status	Published - 1 Jan 1999

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10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

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Van Walree, C. A., Sakuragi, Y., Steensgaard, D. B., Bösinger, C. S., Frigaard, N-U., Cox, R. P., Holzwart, A. R., & Miller, M. (1999). Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides. Photochemistry and Photobiology, 69(3), 322-328. https://doi.org/10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides. / Van Walree, Cornelis A.; Sakuragi, Yumiko; Steensgaard, Dorte B. et al.

In: Photochemistry and Photobiology, Vol. 69, No. 3, 01.01.1999, p. 322-328.

Research output: Contribution to journal › Journal article › Research › peer-review

Van Walree, CA, Sakuragi, Y, Steensgaard, DB, Bösinger, CS, Frigaard, N-U, Cox, RP, Holzwart, AR & Miller, M 1999, 'Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides', Photochemistry and Photobiology, vol. 69, no. 3, pp. 322-328. https://doi.org/10.1562/0031-8655(1999)069<0322:EOATOB>2.3.CO;2

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abstract = "Chlorosomes isolated from two types of green sulfur bacteria, Chlorobium tepidum which contains bacteriochlorophyll c (BChl c) and the BChl e-containing Chlorobium phaeobacteroides, were subjected to alkaline treatment (pH 12.7 at 40°C for 20 min). This caused selective degradation of BChl a, whereas BChl c or e were not affected. Chlorobiumquinone in the chlorosomes was partially degraded by the alkaline treatment but menaquinone was unchanged. Fluorescence decay kinetics showed that alkaline treatment disrupted energy transfer from BChl c or e to BChl a under reducing conditions. However, this did not give rise to any substantial increase in the excited state lifetime of BChl e in C. phaeobacteroides chlorosomes, while for C. tepidum a decrease in the BChl c lifetime was found. The steady-state fluorescence of chlorosomes is highly dependent on the redox potential such that emission is quenched in oxidizing environments. Alkaline treatment diminished this quenching effect and caused a doubling in the BChl c or e emission intensity under aerobic conditions. Single-photon timing experiments confirmed that alkaline treatment inhibits the energy trapping process operative under aerobic conditions. These effects of alkaline treatment on the fluorescence intensity and decay kinetics are likely to be related to the depletion in BChI a or in Chlorobiumquinone or a combination of these.",

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AU - Van Walree, Cornelis A.

AU - Sakuragi, Yumiko

AU - Steensgaard, Dorte B.

AU - Bösinger, Carola S.

AU - Frigaard, Niels-Ulrik

AU - Cox, Raymond P.

AU - Holzwart, Alfred R.

AU - Miller, Mette

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N2 - Chlorosomes isolated from two types of green sulfur bacteria, Chlorobium tepidum which contains bacteriochlorophyll c (BChl c) and the BChl e-containing Chlorobium phaeobacteroides, were subjected to alkaline treatment (pH 12.7 at 40°C for 20 min). This caused selective degradation of BChl a, whereas BChl c or e were not affected. Chlorobiumquinone in the chlorosomes was partially degraded by the alkaline treatment but menaquinone was unchanged. Fluorescence decay kinetics showed that alkaline treatment disrupted energy transfer from BChl c or e to BChl a under reducing conditions. However, this did not give rise to any substantial increase in the excited state lifetime of BChl e in C. phaeobacteroides chlorosomes, while for C. tepidum a decrease in the BChl c lifetime was found. The steady-state fluorescence of chlorosomes is highly dependent on the redox potential such that emission is quenched in oxidizing environments. Alkaline treatment diminished this quenching effect and caused a doubling in the BChl c or e emission intensity under aerobic conditions. Single-photon timing experiments confirmed that alkaline treatment inhibits the energy trapping process operative under aerobic conditions. These effects of alkaline treatment on the fluorescence intensity and decay kinetics are likely to be related to the depletion in BChI a or in Chlorobiumquinone or a combination of these.

AB - Chlorosomes isolated from two types of green sulfur bacteria, Chlorobium tepidum which contains bacteriochlorophyll c (BChl c) and the BChl e-containing Chlorobium phaeobacteroides, were subjected to alkaline treatment (pH 12.7 at 40°C for 20 min). This caused selective degradation of BChl a, whereas BChl c or e were not affected. Chlorobiumquinone in the chlorosomes was partially degraded by the alkaline treatment but menaquinone was unchanged. Fluorescence decay kinetics showed that alkaline treatment disrupted energy transfer from BChl c or e to BChl a under reducing conditions. However, this did not give rise to any substantial increase in the excited state lifetime of BChl e in C. phaeobacteroides chlorosomes, while for C. tepidum a decrease in the BChl c lifetime was found. The steady-state fluorescence of chlorosomes is highly dependent on the redox potential such that emission is quenched in oxidizing environments. Alkaline treatment diminished this quenching effect and caused a doubling in the BChl c or e emission intensity under aerobic conditions. Single-photon timing experiments confirmed that alkaline treatment inhibits the energy trapping process operative under aerobic conditions. These effects of alkaline treatment on the fluorescence intensity and decay kinetics are likely to be related to the depletion in BChI a or in Chlorobiumquinone or a combination of these.

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Effect of alkaline treatment on bacteriochlorophyll a, quinones and energy transfer in chlorosomes from Chlorobium tepidum and Chlorobium phaeobacteroides

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