Diversity of Francisella tularensis Schu4 antigens recognized by T lymphocytes after natural infections in humans: identification of candidate epitopes for inclusion in a rationally designed tularemia vaccine

Julie A McMurry; Stephen H Gregory; Leonard Moise; Daniel Rivera; Soren Buus; Anne S De Groot

doi:10.1016/j.vaccine.2007.01.039

Diversity of Francisella tularensis Schu4 antigens recognized by T lymphocytes after natural infections in humans: identification of candidate epitopes for inclusion in a rationally designed tularemia vaccine

Julie A McMurry, Stephen H Gregory, Leonard Moise, Daniel Rivera, Soren Buus, Anne S De Groot

Department of Immunology and Microbiology

58 Citations (Scopus)

Abstract

The T lymphocyte antigens, which may have a role in protection against tularemia, were predicted by immunoinformatics analysis of Francisella tularensis Schu4. Twenty-seven class II putative promiscuous epitopes and 125 putative class I supertype epitopes were chosen for synthesis; peptides were tested in vitro for their ability to bind HLA and to induce immune responses from PBMCs of 23 previously infected subjects. While the immune responses of individual subjects showed heterogeneity, 95% of the subjects responded strongly to a pool of 27 promiscuous peptides; 25%, 33%, and 44% of subjects responded to pools of 25 A2, A24, and B7 peptides, respectively. These data can aid in the development of novel epitope-based and subunit tularemia vaccines.

Original language	English
Journal	Vaccine
Volume	25
Issue number	16
Pages (from-to)	3179-91
Number of pages	12
ISSN	0264-410X
DOIs	https://doi.org/10.1016/j.vaccine.2007.01.039
Publication status	Published - 2007

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McMurry, J. A., Gregory, S. H., Moise, L., Rivera, D., Buus, S., & De Groot, A. S. (2007). Diversity of Francisella tularensis Schu4 antigens recognized by T lymphocytes after natural infections in humans: identification of candidate epitopes for inclusion in a rationally designed tularemia vaccine. Vaccine, 25(16), 3179-91. https://doi.org/10.1016/j.vaccine.2007.01.039

Diversity of Francisella tularensis Schu4 antigens recognized by T lymphocytes after natural infections in humans: identification of candidate epitopes for inclusion in a rationally designed tularemia vaccine. / McMurry, Julie A; Gregory, Stephen H; Moise, Leonard et al.

In: Vaccine, Vol. 25, No. 16, 2007, p. 3179-91.

Research output: Contribution to journal › Journal article › Research › peer-review

McMurry, JA, Gregory, SH, Moise, L, Rivera, D, Buus, S & De Groot, AS 2007, 'Diversity of Francisella tularensis Schu4 antigens recognized by T lymphocytes after natural infections in humans: identification of candidate epitopes for inclusion in a rationally designed tularemia vaccine', Vaccine, vol. 25, no. 16, pp. 3179-91. https://doi.org/10.1016/j.vaccine.2007.01.039

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title = "Diversity of Francisella tularensis Schu4 antigens recognized by T lymphocytes after natural infections in humans: identification of candidate epitopes for inclusion in a rationally designed tularemia vaccine",

abstract = "The T lymphocyte antigens, which may have a role in protection against tularemia, were predicted by immunoinformatics analysis of Francisella tularensis Schu4. Twenty-seven class II putative promiscuous epitopes and 125 putative class I supertype epitopes were chosen for synthesis; peptides were tested in vitro for their ability to bind HLA and to induce immune responses from PBMCs of 23 previously infected subjects. While the immune responses of individual subjects showed heterogeneity, 95% of the subjects responded strongly to a pool of 27 promiscuous peptides; 25%, 33%, and 44% of subjects responded to pools of 25 A2, A24, and B7 peptides, respectively. These data can aid in the development of novel epitope-based and subunit tularemia vaccines.",

author = "McMurry, {Julie A} and Gregory, {Stephen H} and Leonard Moise and Daniel Rivera and Soren Buus and {De Groot}, {Anne S}",

note = "Keywords: Adult; Aged; Aged, 80 and over; Antigens, Bacterial; Bacterial Vaccines; Drug Design; Epitopes; Female; Francisella tularensis; Genes, MHC Class II; Humans; Male; Middle Aged; T-Lymphocytes; Tularemia; Vaccination",

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N1 - Keywords: Adult; Aged; Aged, 80 and over; Antigens, Bacterial; Bacterial Vaccines; Drug Design; Epitopes; Female; Francisella tularensis; Genes, MHC Class II; Humans; Male; Middle Aged; T-Lymphocytes; Tularemia; Vaccination

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