Concomitant lack of MMP9 and uPA disturbs physiological tissue remodeling

TY - JOUR

T1 - Concomitant lack of MMP9 and uPA disturbs physiological tissue remodeling

AU - Lund, Ida K

AU - Nielsen, Boye S

AU - Almholt, Kasper

AU - Rønø, Birgitte

AU - Hald, Andreas

AU - Illemann, Martin

AU - Green, Kirsty A

AU - Christensen, Ib J

AU - Rømer, John

AU - Lund, Leif R

N1 - Copyright © 2011 Elsevier Inc. All rights reserved.

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP9, gelatinase B) have separately been recognized to play important roles in various tissue remodeling processes. In this study, we demonstrate that deficiency for MMP9 in combination with ablation of either uPA- or tissue-type plasminogen activator (tPA)-catalyzed plasminogen activation is critical to accomplish normal gestation in mice. Gestation was also affected by simultaneous lack of MMP9 and the uPA receptor (uPAR). Interestingly, uPA-deficiency additionally exacerbated the effect of MMP9-deficiency on bone growth and an additive effect caused by combined lack in MMP9 and uPA was observed during healing of cutaneous wounds. By comparison, MMP9-deficiency combined with absence of either tPA or uPAR resulted in no significant effect on wound healing, indicating that the role of uPA during wound healing is independent of uPAR, when MMP9 is absent. Notably, compensatory upregulation of uPA activity was seen in wounds from MMP9-deficient mice. Taken together, these studies reveal essential functional dependency between MMP9 and uPA during gestation and tissue repair.

AB - Urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP9, gelatinase B) have separately been recognized to play important roles in various tissue remodeling processes. In this study, we demonstrate that deficiency for MMP9 in combination with ablation of either uPA- or tissue-type plasminogen activator (tPA)-catalyzed plasminogen activation is critical to accomplish normal gestation in mice. Gestation was also affected by simultaneous lack of MMP9 and the uPA receptor (uPAR). Interestingly, uPA-deficiency additionally exacerbated the effect of MMP9-deficiency on bone growth and an additive effect caused by combined lack in MMP9 and uPA was observed during healing of cutaneous wounds. By comparison, MMP9-deficiency combined with absence of either tPA or uPAR resulted in no significant effect on wound healing, indicating that the role of uPA during wound healing is independent of uPAR, when MMP9 is absent. Notably, compensatory upregulation of uPA activity was seen in wounds from MMP9-deficient mice. Taken together, these studies reveal essential functional dependency between MMP9 and uPA during gestation and tissue repair.

U2 - 10.1016/j.ydbio.2011.07.021

DO - 10.1016/j.ydbio.2011.07.021

M3 - Journal article

SN - 1574-3349

VL - 358

SP - 56

EP - 67

JO - Advances in Developmental Biology

JF - Advances in Developmental Biology

IS - 1

ER -