Characterization and transcriptional analysis of two gene clusters for type IV secretion machinery in Wolbachia of Armadillidium vulgare

Christine Félix; Samuel Pichon; Christine Braquart-Varnier; Henk Braig; Lanming Chen; Roger A Garrett; Gilbert Martin; Pierre Grève

doi:10.1016/j.resmic.2008.05.007

Characterization and transcriptional analysis of two gene clusters for type IV secretion machinery in Wolbachia of Armadillidium vulgare

Christine Félix, Samuel Pichon, Christine Braquart-Varnier, Henk Braig, Lanming Chen, Roger A Garrett, Gilbert Martin, Pierre Grève

Functional Genomics

17 Citations (Scopus)

Abstract

Wolbachia are maternally inherited alpha-proteobacteria that induce feminization of genetic males in most terrestrial crustacean isopods. Two clusters of vir genes for a type IV secretion machinery have been identified at two separate loci and characterized for the first time in a feminizing Wolbachia. Furthermore, we demonstrated that these operons are transcriptionally active in ovaries and in all other tissues tested, suggesting that T4SS has a significant role in Wolbachia biology. These observations and the identification of homologous vir genes in Wolbachia strains infecting insects or nematodes show that vir genes are conserved among Wolbachia strains whatever the phenotype induced by the bacteria.

Original language	English
Journal	Research in Microbiology
Volume	159
Issue number	6
Pages (from-to)	481-5
Number of pages	4
ISSN	0923-2508
DOIs	https://doi.org/10.1016/j.resmic.2008.05.007
Publication status	Published - 2008

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title = "Characterization and transcriptional analysis of two gene clusters for type IV secretion machinery in Wolbachia of Armadillidium vulgare",

abstract = "Wolbachia are maternally inherited alpha-proteobacteria that induce feminization of genetic males in most terrestrial crustacean isopods. Two clusters of vir genes for a type IV secretion machinery have been identified at two separate loci and characterized for the first time in a feminizing Wolbachia. Furthermore, we demonstrated that these operons are transcriptionally active in ovaries and in all other tissues tested, suggesting that T4SS has a significant role in Wolbachia biology. These observations and the identification of homologous vir genes in Wolbachia strains infecting insects or nematodes show that vir genes are conserved among Wolbachia strains whatever the phenotype induced by the bacteria.",

author = "Christine F{\'e}lix and Samuel Pichon and Christine Braquart-Varnier and Henk Braig and Lanming Chen and Garrett, {Roger A} and Gilbert Martin and Pierre Gr{\`e}ve",

note = "Keywords: Animals; Bacterial Proteins; Biological Transport; Female; Feminization; Isopoda; Male; Molecular Sequence Data; Multigene Family; Transcription, Genetic; Wolbachia",

year = "2008",

doi = "10.1016/j.resmic.2008.05.007",

language = "English",

volume = "159",

pages = "481--5",

journal = "Research in Microbiology",

issn = "0923-2508",

publisher = "Elsevier Masson",

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TY - JOUR

T1 - Characterization and transcriptional analysis of two gene clusters for type IV secretion machinery in Wolbachia of Armadillidium vulgare

AU - Félix, Christine

AU - Pichon, Samuel

AU - Braquart-Varnier, Christine

AU - Braig, Henk

AU - Chen, Lanming

AU - Garrett, Roger A

AU - Martin, Gilbert

AU - Grève, Pierre

N1 - Keywords: Animals; Bacterial Proteins; Biological Transport; Female; Feminization; Isopoda; Male; Molecular Sequence Data; Multigene Family; Transcription, Genetic; Wolbachia

PY - 2008

Y1 - 2008

N2 - Wolbachia are maternally inherited alpha-proteobacteria that induce feminization of genetic males in most terrestrial crustacean isopods. Two clusters of vir genes for a type IV secretion machinery have been identified at two separate loci and characterized for the first time in a feminizing Wolbachia. Furthermore, we demonstrated that these operons are transcriptionally active in ovaries and in all other tissues tested, suggesting that T4SS has a significant role in Wolbachia biology. These observations and the identification of homologous vir genes in Wolbachia strains infecting insects or nematodes show that vir genes are conserved among Wolbachia strains whatever the phenotype induced by the bacteria.

AB - Wolbachia are maternally inherited alpha-proteobacteria that induce feminization of genetic males in most terrestrial crustacean isopods. Two clusters of vir genes for a type IV secretion machinery have been identified at two separate loci and characterized for the first time in a feminizing Wolbachia. Furthermore, we demonstrated that these operons are transcriptionally active in ovaries and in all other tissues tested, suggesting that T4SS has a significant role in Wolbachia biology. These observations and the identification of homologous vir genes in Wolbachia strains infecting insects or nematodes show that vir genes are conserved among Wolbachia strains whatever the phenotype induced by the bacteria.

U2 - 10.1016/j.resmic.2008.05.007

DO - 10.1016/j.resmic.2008.05.007

M3 - Journal article

C2 - 18582562

SN - 0923-2508

VL - 159

SP - 481

EP - 485

JO - Research in Microbiology

JF - Research in Microbiology

IS - 6

ER -

Characterization and transcriptional analysis of two gene clusters for type IV secretion machinery in Wolbachia of Armadillidium vulgare

Abstract

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