Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women

Katrien Koppo; Michaela Siklová-Vitková; Eva Klimcáková; Jan Polák; Marie A Marques; Michel Berlan; Johan Van de Voorde; Jens Bülow; Dominique Langin; Isabelle de Glisezinski; Vladimir Stich

doi:10.1152/ajpendo.00240.2011

Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women

Katrien Koppo, Michaela Siklová-Vitková, Eva Klimcáková, Jan Polák, Marie A Marques, Michel Berlan, Johan Van de Voorde, Jens Bülow, Dominique Langin, Isabelle de Glisezinski, Vladimir Stich

Endocrinology and Metabolism

8 Citations (Scopus)

Abstract

The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the α(2)-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found.

Original language	English
Journal	American Journal of Physiology: Endocrinology and Metabolism
Volume	302
Issue number	2
Pages (from-to)	E226-32
ISSN	0193-1849
DOIs	https://doi.org/10.1152/ajpendo.00240.2011
Publication status	Published - 15 Jan 2012

Keywords

Adipose Tissue
Adult
Caloric Restriction
Catecholamines
Diet, Reducing
Female
Glucose Clamp Technique
Humans
Insulin
Insulin Resistance
Lipolysis
Obesity
Weight Loss

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1152/ajpendo.00240.2011

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Koppo, K., Siklová-Vitková, M., Klimcáková, E., Polák, J., Marques, M. A., Berlan, M., Van de Voorde, J., Bülow, J., Langin, D., de Glisezinski, I., & Stich, V. (2012). Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women. American Journal of Physiology: Endocrinology and Metabolism, 302(2), E226-32. https://doi.org/10.1152/ajpendo.00240.2011

Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women. / Koppo, Katrien; Siklová-Vitková, Michaela; Klimcáková, Eva et al.

In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 302, No. 2, 15.01.2012, p. E226-32.

Research output: Contribution to journal › Journal article › Research › peer-review

Koppo, K, Siklová-Vitková, M, Klimcáková, E, Polák, J, Marques, MA, Berlan, M, Van de Voorde, J, Bülow, J, Langin, D, de Glisezinski, I & Stich, V 2012, 'Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women', American Journal of Physiology: Endocrinology and Metabolism, vol. 302, no. 2, pp. E226-32. https://doi.org/10.1152/ajpendo.00240.2011

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abstract = "The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the α(2)-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found.",

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T1 - Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women

AU - Koppo, Katrien

AU - Siklová-Vitková, Michaela

AU - Klimcáková, Eva

AU - Polák, Jan

AU - Marques, Marie A

AU - Berlan, Michel

AU - Van de Voorde, Johan

AU - Bülow, Jens

AU - Langin, Dominique

AU - de Glisezinski, Isabelle

AU - Stich, Vladimir

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N2 - The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the α(2)-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found.

AB - The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the α(2)-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found.

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KW - Adult

KW - Caloric Restriction

KW - Catecholamines

KW - Diet, Reducing

KW - Female

KW - Glucose Clamp Technique

KW - Humans

KW - Insulin

KW - Insulin Resistance

KW - Lipolysis

KW - Obesity

KW - Weight Loss

U2 - 10.1152/ajpendo.00240.2011

DO - 10.1152/ajpendo.00240.2011

M3 - Journal article

C2 - 22028414

SN - 0193-1849

VL - 302

SP - E226-32

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

IS - 2

ER -

Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women

Abstract

Keywords

UN SDGs

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