Abstract
In acute stages of ulcerative colitis (UC), neutrophils migrate from the circulation into inflamed colonic tissue, initiated by yet unknown stimuli. The bacterial peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) is a component of the surface membrane of colonic bacteria such as Escherichia coli and stimulates Ca2+ influx into neutrophils, reflecting the fact that ionized calcium is an important secondary messenger for several neutrophil functions, including locomotion, phagocytosis and free oxygen radical production. Recent studies have revealed that Ca2+ dependent ICAM-1/beta 2-integrin mediated neutrophil migration is impaired in UC patients. The aim of the present work was to study the influx of Ca2+ into peripheral blood neutrophils of UC patients after exposure to FMLP and after binding of either beta 2-integrins or intercellular adhesion molecule-1 (ICAM-1).
| Original language | English |
|---|---|
| Journal | European Journal of Gastroenterology and Hepatology |
| Volume | 15 |
| Issue number | 3 |
| Pages (from-to) | 267-73 |
| Number of pages | 7 |
| ISSN | 0954-691X |
| DOIs | |
| Publication status | Published - 1 Mar 2003 |
Keywords
- Adult
- Aged
- Antigens, CD11
- Calcium
- Cell Adhesion Molecules
- Cell Movement
- Cells, Cultured
- Colitis, Ulcerative
- Cytosol
- Humans
- Male
- Middle Aged
- N-Formylmethionine Leucyl-Phenylalanine
- Neutrophils
- Time Factors
- Up-Regulation