TY - JOUR
T1 - Approaches for cytogenetic and molecular analyses of small flow-sorted cell populations from childhood leukemia bone marrow samples
AU - Obro, Nina Friesgaard
AU - Madsen, Hans Ole
AU - Ryder, Lars Peter
AU - Andersen, Mette Klarskov
AU - Schmiegelow, Kjeld
AU - Marquart, Hanne Vibeke
N1 - Copyright © 2011 Elsevier B.V. All rights reserved.
PY - 2011/6/30
Y1 - 2011/6/30
N2 - Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.
AB - Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.
U2 - 10.1016/j.jim.2011.04.007
DO - 10.1016/j.jim.2011.04.007
M3 - Journal article
SN - 0022-1759
VL - 369
SP - 69
EP - 73
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -