Apoptosis induction by Maackia amurensis agglutinin in childhood acute lymphoblastic leukemic cells.

Sarika Kapoor; Ram Marwaha; Siddhartha Majumdar; Sujata Ghosh

doi:10.1016/j.leukres.2007.08.007

Apoptosis induction by Maackia amurensis agglutinin in childhood acute lymphoblastic leukemic cells.

Sarika Kapoor, Ram Marwaha, Siddhartha Majumdar, Sujata Ghosh

Nutritional Immunology

9 Citations (Scopus)

Abstract

Malignant transformation is known to be associated with changes in cell surface carbohydrate-architecture, which can be detected by lectins. In the present study, Maackia amurensis agglutinin (MAA), specific for NeuNAcalpha(2-->3)Gal/GalNAc showed strong binding with lymphoblasts of children having acute lymphoblastic leukemia (ALL) as compared to cells from children with non-hematological disorders ("Controls"). MAA recognized a 66 kDa sialoglycoprotein present in membrane fraction of ALL cells. Moreover, MAA induced apoptosis in ALL cells was found to be reduced significantly in presence of GM2/IgG(MAA). Thus, MAA has a potential to be used as diagnostic and therapeutic agent in case of childhood-ALL.

Original language	English
Journal	Leukemia research : a Forum for Studies on Leukemia and Normal Hemopoiesis
Volume	32
Issue number	4
Pages (from-to)	559-67
Number of pages	8
ISSN	0145-2126
DOIs	https://doi.org/10.1016/j.leukres.2007.08.007
Publication status	Published - 2007

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10.1016/j.leukres.2007.08.007

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title = "Apoptosis induction by Maackia amurensis agglutinin in childhood acute lymphoblastic leukemic cells.",

abstract = "Malignant transformation is known to be associated with changes in cell surface carbohydrate-architecture, which can be detected by lectins. In the present study, Maackia amurensis agglutinin (MAA), specific for NeuNAcalpha(2-->3)Gal/GalNAc showed strong binding with lymphoblasts of children having acute lymphoblastic leukemia (ALL) as compared to cells from children with non-hematological disorders ({"}Controls{"}). MAA recognized a 66 kDa sialoglycoprotein present in membrane fraction of ALL cells. Moreover, MAA induced apoptosis in ALL cells was found to be reduced significantly in presence of GM2/IgG(MAA). Thus, MAA has a potential to be used as diagnostic and therapeutic agent in case of childhood-ALL.",

author = "Sarika Kapoor and Ram Marwaha and Siddhartha Majumdar and Sujata Ghosh",

note = "Keywords: Apoptosis; Blotting, Western; Cell Line; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Glycoproteins; Humans; Lymphocytes; Male; Neuraminidase; Phytohemagglutinins; Precursor Cell Lymphoblastic Leukemia-Lymphoma",

year = "2007",

doi = "10.1016/j.leukres.2007.08.007",

language = "English",

volume = "32",

pages = "559--67",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Pergamon Press",

number = "4",

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TY - JOUR

T1 - Apoptosis induction by Maackia amurensis agglutinin in childhood acute lymphoblastic leukemic cells.

AU - Kapoor, Sarika

AU - Marwaha, Ram

AU - Majumdar, Siddhartha

AU - Ghosh, Sujata

N1 - Keywords: Apoptosis; Blotting, Western; Cell Line; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Glycoproteins; Humans; Lymphocytes; Male; Neuraminidase; Phytohemagglutinins; Precursor Cell Lymphoblastic Leukemia-Lymphoma

PY - 2007

Y1 - 2007

N2 - Malignant transformation is known to be associated with changes in cell surface carbohydrate-architecture, which can be detected by lectins. In the present study, Maackia amurensis agglutinin (MAA), specific for NeuNAcalpha(2-->3)Gal/GalNAc showed strong binding with lymphoblasts of children having acute lymphoblastic leukemia (ALL) as compared to cells from children with non-hematological disorders ("Controls"). MAA recognized a 66 kDa sialoglycoprotein present in membrane fraction of ALL cells. Moreover, MAA induced apoptosis in ALL cells was found to be reduced significantly in presence of GM2/IgG(MAA). Thus, MAA has a potential to be used as diagnostic and therapeutic agent in case of childhood-ALL.

AB - Malignant transformation is known to be associated with changes in cell surface carbohydrate-architecture, which can be detected by lectins. In the present study, Maackia amurensis agglutinin (MAA), specific for NeuNAcalpha(2-->3)Gal/GalNAc showed strong binding with lymphoblasts of children having acute lymphoblastic leukemia (ALL) as compared to cells from children with non-hematological disorders ("Controls"). MAA recognized a 66 kDa sialoglycoprotein present in membrane fraction of ALL cells. Moreover, MAA induced apoptosis in ALL cells was found to be reduced significantly in presence of GM2/IgG(MAA). Thus, MAA has a potential to be used as diagnostic and therapeutic agent in case of childhood-ALL.

U2 - 10.1016/j.leukres.2007.08.007

DO - 10.1016/j.leukres.2007.08.007

M3 - Journal article

C2 - 17889364

SN - 0145-2126

VL - 32

SP - 559

EP - 567

JO - Leukemia Research

JF - Leukemia Research

IS - 4

ER -

Apoptosis induction by Maackia amurensis agglutinin in childhood acute lymphoblastic leukemic cells.

Abstract

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