An interlaboratory comparison between similar methods for determination of melatonin, cortisol and testosterone in saliva

Marie Aarrebo Jensen; Leen Mortier; Eitetsu Koh; Brian Keevil; Sirpa Hyttinen; Åse Marie Hansen

doi:10.3109/00365513.2014.900693

An interlaboratory comparison between similar methods for determination of melatonin, cortisol and testosterone in saliva

Marie Aarrebo Jensen, Leen Mortier, Eitetsu Koh, Brian Keevil, Sirpa Hyttinen, Åse Marie Hansen

Section of Social Medicine

13 Citations (Scopus)

Abstract

An interlaboratory comparison study for melatonin, cortisol and testosterone in saliva in which five laboratories participated is reported in this study. Each laboratory blindly measured eight samples prepared from natural saliva spiked with melatonin, cortisol and testosterone in the range 0-579 pmol/L for melatonin, 0-90 nmol/L for cortisol, and 0-622 pmol/L for testosterone. The recovery of spiked material for melatonin ranged from 91-110%, from 83-100% for cortisol and from 80-94% for testosterone. The content of natural hormone in saliva was estimated to be between 0.278 and 6.90 pmol/L for melatonin, 0.56 and 6.72 nmol/L for cortisol and 11.9 and 73.8 pmol/L for testosterone. This indicates a large interlaboratory variation. The present study emphasizes the importance of external quality control for the analysis of melatonin, cortisol and testosterone in saliva.

Original language	English
Journal	Scandinavian Journal of Clinical & Laboratory Investigation
Volume	74
Issue number	5
Pages (from-to)	454-461
Number of pages	8
ISSN	0036-5513
DOIs	https://doi.org/10.3109/00365513.2014.900693
Publication status	Published - Aug 2014

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An interlaboratory comparison between similar methods for determination of melatonin, cortisol and testosterone in saliva. / Jensen, Marie Aarrebo; Mortier, Leen; Koh, Eitetsu et al.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 74, No. 5, 08.2014, p. 454-461.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Hyttinen, Sirpa

AU - Hansen, Åse Marie

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An interlaboratory comparison between similar methods for determination of melatonin, cortisol and testosterone in saliva

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