An improved method for the detection of peptide-induced upregulation of HLA-A2 molecules on TAP-deficient T2 cells

TY - JOUR

T1 - An improved method for the detection of peptide-induced upregulation of HLA-A2 molecules on TAP-deficient T2 cells

AU - Regner, M

AU - Claesson, Mogens Helweg

AU - Bregenholt, S

AU - Röpke, M

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Peptide-induced upregulation of MHC class I on TAP-deficient T2 cells is used to monitor peptide binding to class I molecules. We present a temperature-dependent modification of the T2 assay which increases the sensitivity of peptide binding to MHC class I with several orders of magnitude.

AB - Peptide-induced upregulation of MHC class I on TAP-deficient T2 cells is used to monitor peptide binding to class I molecules. We present a temperature-dependent modification of the T2 assay which increases the sensitivity of peptide binding to MHC class I with several orders of magnitude.

KW - ATP-Binding Cassette Transporters

KW - Amino Acid Sequence

KW - Cell Line

KW - HLA-A2 Antigen

KW - Humans

KW - Immunoassay

KW - Major Histocompatibility Complex

KW - Orthomyxoviridae

KW - Peptides

KW - Protein Binding

KW - Tumor Suppressor Protein p53

KW - Up-Regulation

KW - Viral Matrix Proteins

M3 - Journal article

C2 - 8854086

SN - 0254-9670

VL - 13

SP - 30

EP - 35

JO - Experimental and Clinical Immunogenetics

JF - Experimental and Clinical Immunogenetics

IS - 1

ER -