Affinity-purified human interleukin I is cytotoxic to isolated islets of Langerhans

T Mandrup-Poulsen; K Bendtzen; J Nerup; C A Dinarello; M Svenson; Jens Høiriis Nielsen

Affinity-purified human interleukin I is cytotoxic to isolated islets of Langerhans

T Mandrup-Poulsen, K Bendtzen, J Nerup, C A Dinarello, M Svenson, Jens Høiriis Nielsen

296 Citations (Scopus)

Abstract

Addition of highly purified human Interleukin-1 to the culture medium of isolated rat islets of Langerhans for 6 days led to 88% inhibition of glucose-induced insulin-release, reduction of islet contents of insulin and glucagon to 31% and 8% respectively, and disintegration of the islets. These effects were dose-dependent and reproducible when using three different Interleukin-1 preparations. Highly purified human Interleukin-2, Lymphotoxin, Leucocyte Migration Inhibitory Factor and Macrophage Migration Inhibitory Factor were ineffective. These findings suggest that Interleukin-1 may play an important role in the molecular mechanisms underlying autoimmune B-cell destruction leading to Type 1 (insulin-dependent) diabetes mellitus.

Original language	English
Journal	Diabetologia
Volume	29
Issue number	1
Pages (from-to)	63-7
Number of pages	5
ISSN	0012-186X
Publication status	Published - Jan 1986

Keywords

Animals
Glucagon
Humans
Insulin
Interleukin-1
Islets of Langerhans
Male
Rats
Rats, Inbred Strains

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title = "Affinity-purified human interleukin I is cytotoxic to isolated islets of Langerhans",

abstract = "Addition of highly purified human Interleukin-1 to the culture medium of isolated rat islets of Langerhans for 6 days led to 88% inhibition of glucose-induced insulin-release, reduction of islet contents of insulin and glucagon to 31% and 8% respectively, and disintegration of the islets. These effects were dose-dependent and reproducible when using three different Interleukin-1 preparations. Highly purified human Interleukin-2, Lymphotoxin, Leucocyte Migration Inhibitory Factor and Macrophage Migration Inhibitory Factor were ineffective. These findings suggest that Interleukin-1 may play an important role in the molecular mechanisms underlying autoimmune B-cell destruction leading to Type 1 (insulin-dependent) diabetes mellitus.",

keywords = "Animals, Glucagon, Humans, Insulin, Interleukin-1, Islets of Langerhans, Male, Rats, Rats, Inbred Strains",

author = "T Mandrup-Poulsen and K Bendtzen and J Nerup and Dinarello, {C A} and M Svenson and Nielsen, {Jens H{\o}iriis}",

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T1 - Affinity-purified human interleukin I is cytotoxic to isolated islets of Langerhans

AU - Mandrup-Poulsen, T

AU - Bendtzen, K

AU - Nerup, J

AU - Dinarello, C A

AU - Svenson, M

AU - Nielsen, Jens Høiriis

PY - 1986/1

Y1 - 1986/1

N2 - Addition of highly purified human Interleukin-1 to the culture medium of isolated rat islets of Langerhans for 6 days led to 88% inhibition of glucose-induced insulin-release, reduction of islet contents of insulin and glucagon to 31% and 8% respectively, and disintegration of the islets. These effects were dose-dependent and reproducible when using three different Interleukin-1 preparations. Highly purified human Interleukin-2, Lymphotoxin, Leucocyte Migration Inhibitory Factor and Macrophage Migration Inhibitory Factor were ineffective. These findings suggest that Interleukin-1 may play an important role in the molecular mechanisms underlying autoimmune B-cell destruction leading to Type 1 (insulin-dependent) diabetes mellitus.

AB - Addition of highly purified human Interleukin-1 to the culture medium of isolated rat islets of Langerhans for 6 days led to 88% inhibition of glucose-induced insulin-release, reduction of islet contents of insulin and glucagon to 31% and 8% respectively, and disintegration of the islets. These effects were dose-dependent and reproducible when using three different Interleukin-1 preparations. Highly purified human Interleukin-2, Lymphotoxin, Leucocyte Migration Inhibitory Factor and Macrophage Migration Inhibitory Factor were ineffective. These findings suggest that Interleukin-1 may play an important role in the molecular mechanisms underlying autoimmune B-cell destruction leading to Type 1 (insulin-dependent) diabetes mellitus.

KW - Animals

KW - Glucagon

KW - Humans

KW - Insulin

KW - Interleukin-1

KW - Islets of Langerhans

KW - Male

KW - Rats

KW - Rats, Inbred Strains

M3 - Journal article

C2 - 3514344

SN - 0012-186X

VL - 29

SP - 63

EP - 67

JO - Diabetologia

JF - Diabetologia

IS - 1

ER -

Affinity-purified human interleukin I is cytotoxic to isolated islets of Langerhans

Abstract

Keywords

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