松仁蛋白酶解液体外抗氧化活性分析

Xiufang Lin; Xiaohong Wu; Zichen Zhao; Bingxin Xu; Xiaotang Gao; Min Liu

松仁蛋白酶解液体外抗氧化活性分析

Xiufang Lin, Xiaohong Wu, Zichen Zhao, Bingxin Xu, Xiaotang Gao, Min Liu

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Abstract

Enzymatic hydrolysis experiment of pine kernel protein was conducted by two kinds of gastrointestinal digestive enzymes ( pepsin and trypsin ) ， and in vitro antioxidant activities of enzymatic
hydrolysates of pine kernel protein obtained by hydrolysis of pepsin， trypsin and pepsin － trypsin were
studied． The results showed that after enzymatic hydrolysis for 30 min， the pepsin enzymatic hydrolysate
of pine kernel protein had the strongest in vitro antioxidant activity， and the scavenging rates on ·OH
radical and O －
2 ·radical were 67． 43% and 55． 66% respectively; the trypsin enzymatic hydrolysate of
pine kernel protein had the strongest scavenging capacity on ·OH radical after enzymatic hydrolysis for
60 min，and the scavenging rate on ·OH radical was 68． 79% ，while it had the strongest scavenging capacity on O2－ · radical after enzymatic hydrolysis for 120 min， and the scavenging rate on O2－ · radical
was 39． 20% ; pepsin － trypsin step － by － step enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity after enzymatic hydrolysis for 240 min，and the scavenging rates on·OH
radical and O －
2 ·radical were 79． 11% and 68． 99% respectively

Original language	Chinese
Journal	中国油脂
Volume	39
Issue number	11
Pages (from-to)	29-31
Number of pages	3
ISSN	1003-7969
Publication status	Published - 2014

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Cite this

@article{5aa3350d1aaf42008e1f86cbce8e79bb,

title = "松仁蛋白酶解液体外抗氧化活性分析",

abstract = "Enzymatic hydrolysis experiment of pine kernel protein was conducted by two kinds of gastrointestinal digestive enzymes ( pepsin and trypsin ) ， and in vitro antioxidant activities of enzymatichydrolysates of pine kernel protein obtained by hydrolysis of pepsin， trypsin and pepsin － trypsin werestudied． The results showed that after enzymatic hydrolysis for 30 min， the pepsin enzymatic hydrolysateof pine kernel protein had the strongest in vitro antioxidant activity， and the scavenging rates on ·OHradical and O －2 ·radical were 67． 43% and 55． 66% respectively; the trypsin enzymatic hydrolysate ofpine kernel protein had the strongest scavenging capacity on ·OH radical after enzymatic hydrolysis for60 min，and the scavenging rate on ·OH radical was 68． 79% ，while it had the strongest scavenging capacity on O2－ · radical after enzymatic hydrolysis for 120 min， and the scavenging rate on O2－ · radicalwas 39． 20% ; pepsin － trypsin step － by － step enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity after enzymatic hydrolysis for 240 min，and the scavenging rates on·OHradical and O －2 ·radical were 79． 11% and 68． 99% respectively",

author = "Xiufang Lin and {Xiaohong Wu} and Zichen Zhao and Bingxin Xu and Xiaotang Gao and Min Liu",

year = "2014",

language = "Kinesisk",

volume = "39",

pages = "29--31",

journal = "中国油脂",

issn = "1003-7969",

number = "11",

}

TY - JOUR

T1 - 松仁蛋白酶解液体外抗氧化活性分析

AU - Lin, Xiufang

AU - Xiaohong Wu

AU - Zhao, Zichen

AU - Xu, Bingxin

AU - Gao, Xiaotang

AU - Liu, Min

PY - 2014

Y1 - 2014

N2 - Enzymatic hydrolysis experiment of pine kernel protein was conducted by two kinds of gastrointestinal digestive enzymes ( pepsin and trypsin ) ， and in vitro antioxidant activities of enzymatichydrolysates of pine kernel protein obtained by hydrolysis of pepsin， trypsin and pepsin － trypsin werestudied． The results showed that after enzymatic hydrolysis for 30 min， the pepsin enzymatic hydrolysateof pine kernel protein had the strongest in vitro antioxidant activity， and the scavenging rates on ·OHradical and O －2 ·radical were 67． 43% and 55． 66% respectively; the trypsin enzymatic hydrolysate ofpine kernel protein had the strongest scavenging capacity on ·OH radical after enzymatic hydrolysis for60 min，and the scavenging rate on ·OH radical was 68． 79% ，while it had the strongest scavenging capacity on O2－ · radical after enzymatic hydrolysis for 120 min， and the scavenging rate on O2－ · radicalwas 39． 20% ; pepsin － trypsin step － by － step enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity after enzymatic hydrolysis for 240 min，and the scavenging rates on·OHradical and O －2 ·radical were 79． 11% and 68． 99% respectively

AB - Enzymatic hydrolysis experiment of pine kernel protein was conducted by two kinds of gastrointestinal digestive enzymes ( pepsin and trypsin ) ， and in vitro antioxidant activities of enzymatichydrolysates of pine kernel protein obtained by hydrolysis of pepsin， trypsin and pepsin － trypsin werestudied． The results showed that after enzymatic hydrolysis for 30 min， the pepsin enzymatic hydrolysateof pine kernel protein had the strongest in vitro antioxidant activity， and the scavenging rates on ·OHradical and O －2 ·radical were 67． 43% and 55． 66% respectively; the trypsin enzymatic hydrolysate ofpine kernel protein had the strongest scavenging capacity on ·OH radical after enzymatic hydrolysis for60 min，and the scavenging rate on ·OH radical was 68． 79% ，while it had the strongest scavenging capacity on O2－ · radical after enzymatic hydrolysis for 120 min， and the scavenging rate on O2－ · radicalwas 39． 20% ; pepsin － trypsin step － by － step enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity after enzymatic hydrolysis for 240 min，and the scavenging rates on·OHradical and O －2 ·radical were 79． 11% and 68． 99% respectively

M3 - Tidsskriftartikel

SN - 1003-7969

VL - 39

SP - 29

EP - 31

JO - 中国油脂

JF - 中国油脂

IS - 11

ER -