Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons

Åse Marie Hansen; I L Olsen; E Holst; O M Poulsen

Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons

Åse Marie Hansen, I L Olsen, E Holst, O M Poulsen

Institut for Folkesundhedsvidenskab

30 Citationer (Scopus)

Abstract

A high-performance liquid chromatography/fluorescence method using multiple wavelength shift for simultaneous quantification of different PAH compounds was developed. The new method was superior to the methods of DONG and GREENBERG [J. Liquid Chromatogr. 11, 1887-1905 (1988)] and WISE et al. [Polycyclic aromat. Hydrocarb. (in press)] with respect to sensitivity of detection of the majority of 15 PAH compounds, and in particular of naphthalene, acenaphthene, fluorene and benzo(b)fluoranthene. The method of validation analysis employed showed that the new method is in statistical balance meaning that no systematic errors, and only small unsystematic errors, could be demonstrated. Furthermore, the method had a good reproducibility and a high sensitivity.

Originalsprog	Engelsk
Tidsskrift	Annals of Occupational Hygiene
Vol/bind	35
Udgave nummer	6
Sider (fra-til)	603-11
Antal sider	9
ISSN	2398-7308
Status	Udgivet - dec. 1991

Citationsformater

Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons. / Hansen, Åse Marie; Olsen, I L; Holst, E et al.

I: Annals of Occupational Hygiene, Bind 35, Nr. 6, 12.1991, s. 603-11.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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abstract = "A high-performance liquid chromatography/fluorescence method using multiple wavelength shift for simultaneous quantification of different PAH compounds was developed. The new method was superior to the methods of DONG and GREENBERG [J. Liquid Chromatogr. 11, 1887-1905 (1988)] and WISE et al. [Polycyclic aromat. Hydrocarb. (in press)] with respect to sensitivity of detection of the majority of 15 PAH compounds, and in particular of naphthalene, acenaphthene, fluorene and benzo(b)fluoranthene. The method of validation analysis employed showed that the new method is in statistical balance meaning that no systematic errors, and only small unsystematic errors, could be demonstrated. Furthermore, the method had a good reproducibility and a high sensitivity.",

keywords = "Air Pollutants, Occupational, Chromatography, High Pressure Liquid, Evaluation Studies as Topic, Fluorescent Dyes, Humans, Polycyclic Compounds, Reproducibility of Results",

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T1 - Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons

AU - Hansen, Åse Marie

AU - Olsen, I L

AU - Holst, E

AU - Poulsen, O M

PY - 1991/12

Y1 - 1991/12

N2 - A high-performance liquid chromatography/fluorescence method using multiple wavelength shift for simultaneous quantification of different PAH compounds was developed. The new method was superior to the methods of DONG and GREENBERG [J. Liquid Chromatogr. 11, 1887-1905 (1988)] and WISE et al. [Polycyclic aromat. Hydrocarb. (in press)] with respect to sensitivity of detection of the majority of 15 PAH compounds, and in particular of naphthalene, acenaphthene, fluorene and benzo(b)fluoranthene. The method of validation analysis employed showed that the new method is in statistical balance meaning that no systematic errors, and only small unsystematic errors, could be demonstrated. Furthermore, the method had a good reproducibility and a high sensitivity.

AB - A high-performance liquid chromatography/fluorescence method using multiple wavelength shift for simultaneous quantification of different PAH compounds was developed. The new method was superior to the methods of DONG and GREENBERG [J. Liquid Chromatogr. 11, 1887-1905 (1988)] and WISE et al. [Polycyclic aromat. Hydrocarb. (in press)] with respect to sensitivity of detection of the majority of 15 PAH compounds, and in particular of naphthalene, acenaphthene, fluorene and benzo(b)fluoranthene. The method of validation analysis employed showed that the new method is in statistical balance meaning that no systematic errors, and only small unsystematic errors, could be demonstrated. Furthermore, the method had a good reproducibility and a high sensitivity.

KW - Air Pollutants, Occupational

KW - Chromatography, High Pressure Liquid

KW - Evaluation Studies as Topic

KW - Fluorescent Dyes

KW - Humans

KW - Polycyclic Compounds

KW - Reproducibility of Results

M3 - Journal article

C2 - 1768009

SN - 2398-7308

VL - 35

SP - 603

EP - 611

JO - Annals of Occupational Hygiene

JF - Annals of Occupational Hygiene

IS - 6

ER -

Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons

Abstract

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