@inbook{245502c71ab44ccca0bd38eaa7b25d57,
title = "USER-derived cloning methods and their primer design",
abstract = "Uracil excision-based cloning through USER{\texttrademark} (Uracil-Specific Excision Reagent) is an efficient ligase-free cloning technique that comprises USER cloning, USER fusion, and USER cassette-free (UCF) USER fusion. These USER-derived cloning techniques enable seamless assembly of multiple DNA fragments in one construct. Though governed by a few simple rules primer design for USER-based fusion of PCR fragments can prove time-consuming for inexperienced users. The Primer Help for USER (PHUSER) software is an easy-to-use primer design tool for USER-based methods. In this chapter, we present a PHUSER software protocol for designing primers for USER-derived cloning techniques.",
author = "Bo Salomonsen and Mortensen, {Uffe Hasbro} and Halkier, {Barbara Ann}",
year = "2014",
doi = "10.1007/978-1-62703-764-8_5",
language = "English",
isbn = "978-1-62703-763-1",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "59--72",
booktitle = "DNA cloning and assembly methods",
address = "United States",
}