Type III CRISPR-Cas System: Introduction And Its Application for Genetic Manipulations

Tao Liu; Saifu Pan; Yingjun Li; Nan Peng; Qunxin She

doi:10.21775/cimb.026.001

Type III CRISPR-Cas System: Introduction And Its Application for Genetic Manipulations

Tao Liu, Saifu Pan, Yingjun Li, Nan Peng, Qunxin She

Functional genomics

7 Citationer (Scopus)

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes provide adaptive immunity against invasion of foreign nucleic acids in archaea and bacteria. The system functions in three distinct stages: adaptation, biogenesis, and interference. CRISPR-Cas systems are currently classified into at least five different types, each with a signature protein among which Type III systems exhibit a dual DNA/RNA interference activity. Structures of a few Type III surveillance complexes have been determined: they are composed of several different subunits and exhibit striking architectural similarities to Type I surveillance complexes. Here, we review the genetic, biochemical, and structural studies concerning CRISPR-Cas Type III systems and discuss their application for genetic manipulations, including genome engineering and gene silencing.

Originalsprog	Engelsk
Tidsskrift	Current Issues in Molecular Biology
Vol/bind	26
Sider (fra-til)	1-14
Antal sider	14
ISSN	1467-3037
DOI	https://doi.org/10.21775/cimb.026.001
Status	Udgivet - 1 jan. 2018

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10.21775/cimb.026.001Licens: CC BY

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T2 - Introduction And Its Application for Genetic Manipulations

AU - Liu, Tao

AU - Pan, Saifu

AU - Li, Yingjun

AU - Peng, Nan

AU - She, Qunxin

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes provide adaptive immunity against invasion of foreign nucleic acids in archaea and bacteria. The system functions in three distinct stages: adaptation, biogenesis, and interference. CRISPR-Cas systems are currently classified into at least five different types, each with a signature protein among which Type III systems exhibit a dual DNA/RNA interference activity. Structures of a few Type III surveillance complexes have been determined: they are composed of several different subunits and exhibit striking architectural similarities to Type I surveillance complexes. Here, we review the genetic, biochemical, and structural studies concerning CRISPR-Cas Type III systems and discuss their application for genetic manipulations, including genome engineering and gene silencing.

AB - Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes provide adaptive immunity against invasion of foreign nucleic acids in archaea and bacteria. The system functions in three distinct stages: adaptation, biogenesis, and interference. CRISPR-Cas systems are currently classified into at least five different types, each with a signature protein among which Type III systems exhibit a dual DNA/RNA interference activity. Structures of a few Type III surveillance complexes have been determined: they are composed of several different subunits and exhibit striking architectural similarities to Type I surveillance complexes. Here, we review the genetic, biochemical, and structural studies concerning CRISPR-Cas Type III systems and discuss their application for genetic manipulations, including genome engineering and gene silencing.

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JO - Current Issues in Molecular Biology

JF - Current Issues in Molecular Biology

ER -

Type III CRISPR-Cas System: Introduction And Its Application for Genetic Manipulations

Abstract

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