Truncated glucagon-like peptide-1 (proglucagon 78-107 amide), an intestinal insulin-releasing peptide, has specific receptors on rat insulinoma cells (RIN 5AH)

C Orskov; Jens Høiriis Nielsen

Truncated glucagon-like peptide-1 (proglucagon 78-107 amide), an intestinal insulin-releasing peptide, has specific receptors on rat insulinoma cells (RIN 5AH)

Inflammation, Metabolism and Oxidation

41 Citationer (Scopus)

Abstract

We studied binding of 125I-labelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide) to a cloned rat insulin-producing cell line, RIN 5AH, in monolayer culture. Interaction of the peptide with pancreatic insulinoma cells was saturable and time dependent. Half-maximal binding was obtained when the cells were incubated in the presence of 3.3 x 10(-9) mol/l unlabelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide). Neither glucagon, full-length glucagon-like peptide-1 (proglucagon 72-107 amide) nor gastric inhibitory peptide competed for binding in concentrations up to 10(-6) mol/l.

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	229
Udgave nummer	1
Sider (fra-til)	175-8
Antal sider	4
ISSN	0014-5793
Status	Udgivet - 29 feb. 1988

Citationsformater

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abstract = "We studied binding of 125I-labelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide) to a cloned rat insulin-producing cell line, RIN 5AH, in monolayer culture. Interaction of the peptide with pancreatic insulinoma cells was saturable and time dependent. Half-maximal binding was obtained when the cells were incubated in the presence of 3.3 x 10(-9) mol/l unlabelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide). Neither glucagon, full-length glucagon-like peptide-1 (proglucagon 72-107 amide) nor gastric inhibitory peptide competed for binding in concentrations up to 10(-6) mol/l.",

keywords = "Adenoma, Islet Cell, Animals, Binding, Competitive, Glucagon, Glucagon-Like Peptide 1, Insulin, Insulinoma, Intestines, Kinetics, Pancreatic Neoplasms, Peptides, Rats, Receptors, Cell Surface, Receptors, Glucagon",

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T1 - Truncated glucagon-like peptide-1 (proglucagon 78-107 amide), an intestinal insulin-releasing peptide, has specific receptors on rat insulinoma cells (RIN 5AH)

AU - Orskov, C

AU - Nielsen, Jens Høiriis

PY - 1988/2/29

Y1 - 1988/2/29

N2 - We studied binding of 125I-labelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide) to a cloned rat insulin-producing cell line, RIN 5AH, in monolayer culture. Interaction of the peptide with pancreatic insulinoma cells was saturable and time dependent. Half-maximal binding was obtained when the cells were incubated in the presence of 3.3 x 10(-9) mol/l unlabelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide). Neither glucagon, full-length glucagon-like peptide-1 (proglucagon 72-107 amide) nor gastric inhibitory peptide competed for binding in concentrations up to 10(-6) mol/l.

AB - We studied binding of 125I-labelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide) to a cloned rat insulin-producing cell line, RIN 5AH, in monolayer culture. Interaction of the peptide with pancreatic insulinoma cells was saturable and time dependent. Half-maximal binding was obtained when the cells were incubated in the presence of 3.3 x 10(-9) mol/l unlabelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide). Neither glucagon, full-length glucagon-like peptide-1 (proglucagon 72-107 amide) nor gastric inhibitory peptide competed for binding in concentrations up to 10(-6) mol/l.

KW - Adenoma, Islet Cell

KW - Animals

KW - Binding, Competitive

KW - Glucagon

KW - Glucagon-Like Peptide 1

KW - Insulin

KW - Insulinoma

KW - Intestines

KW - Kinetics

KW - Pancreatic Neoplasms

KW - Peptides

KW - Rats

KW - Receptors, Cell Surface

KW - Receptors, Glucagon

M3 - Journal article

C2 - 2831085

SN - 0014-5793

VL - 229

SP - 175

EP - 178

JO - F E B S Letters

JF - F E B S Letters

IS - 1

ER -

Truncated glucagon-like peptide-1 (proglucagon 78-107 amide), an intestinal insulin-releasing peptide, has specific receptors on rat insulinoma cells (RIN 5AH)

Abstract

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