Tools to discover anionic and nonionic polyfluorinated alkyl surfactants by liquid chromatography electrospray ionisation mass spectrometry

TY - JOUR

T1 - Tools to discover anionic and nonionic polyfluorinated alkyl surfactants by liquid chromatography electrospray ionisation mass spectrometry

AU - Trier, Xenia

AU - Granby, Kit

AU - Christensen, Jan H.

PY - 2011/10/7

Y1 - 2011/10/7

N2 - A tiered approach is proposed for the discovery of unknown anionic and nonionic polyfluorinated alkyl surfactants (PFASs) by reversed phase ultra high performance liquid chromatography (UHPLC) - negative electrospray ionisation - quadrupole time of flight mass spectrometry (UHPLC-ESI --QTOF-MS). The chromatographic separation, ionisation and detection of PFASs mixtures, was achieved at high pH (pH=9.7) with NH 4OH as additive. To distinguish PFASs from other chemicals we used the characteristic negative mass defects of PFASs, their specific losses of 20Da (HF) and the presence of series of chromatographic peaks, belonging to homologues series with m/z of n×50Da (CF 2) or n×100Da (CF 2CF 2). The elemental composition of the precursor ions were deducted from the accurate m/z values of the deprotonated molecules [M-H] -. In case of in-source fragmentation, the presence of dimers, e.g. [M 2-H] - and adduct ions such as [M-H+solvent] - and [(M-H)(M-H+Na) n] - were used to confirm the identity of the precursor ions. In relation to quantification of PFASs, we discuss how their surfactancy influence the ESI processes, challenge their handling in solution and choices of precursor-to-product ions for MSMS of e.g., structural PFAS isomers. The method has been used to discover PFASs in industrial blends and in extracts from food contact materials.

AB - A tiered approach is proposed for the discovery of unknown anionic and nonionic polyfluorinated alkyl surfactants (PFASs) by reversed phase ultra high performance liquid chromatography (UHPLC) - negative electrospray ionisation - quadrupole time of flight mass spectrometry (UHPLC-ESI --QTOF-MS). The chromatographic separation, ionisation and detection of PFASs mixtures, was achieved at high pH (pH=9.7) with NH 4OH as additive. To distinguish PFASs from other chemicals we used the characteristic negative mass defects of PFASs, their specific losses of 20Da (HF) and the presence of series of chromatographic peaks, belonging to homologues series with m/z of n×50Da (CF 2) or n×100Da (CF 2CF 2). The elemental composition of the precursor ions were deducted from the accurate m/z values of the deprotonated molecules [M-H] -. In case of in-source fragmentation, the presence of dimers, e.g. [M 2-H] - and adduct ions such as [M-H+solvent] - and [(M-H)(M-H+Na) n] - were used to confirm the identity of the precursor ions. In relation to quantification of PFASs, we discuss how their surfactancy influence the ESI processes, challenge their handling in solution and choices of precursor-to-product ions for MSMS of e.g., structural PFAS isomers. The method has been used to discover PFASs in industrial blends and in extracts from food contact materials.

U2 - 10.1016/j.chroma.2011.07.057

DO - 10.1016/j.chroma.2011.07.057

M3 - Journal article

SN - 0301-4770

VL - 1218

SP - 7094

EP - 7104

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 40

ER -