The non-coding RNA Ncr0700/PmgR1 is required for photomixotrophic growth and the regulation of glycogen accumulation in the cyanobacterium Synechocystis sp. PCC 6803

TY - JOUR

T1 - The non-coding RNA Ncr0700/PmgR1 is required for photomixotrophic growth and the regulation of glycogen accumulation in the cyanobacterium Synechocystis sp. PCC 6803

AU - de Porcellinis, Alice Jara

AU - Klähn, Stephan

AU - Rosgaard, Lisa

AU - Kirsch, Rebekka

AU - Gutekunst, Kirstin

AU - Georg, Jens

AU - Hess, Wolfgang R.

AU - Sakuragi, Yumiko

N1 - © The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: [email protected].

PY - 2016/10

Y1 - 2016/10

N2 - Carbohydrate metabolism is a tightly regulated process in photosynthetic organisms. In the cyanobacterium Synechocystis sp. PCC 6803, the photomixotrophic growth protein A (PmgA) is involved in the regulation of glucose and storage carbohydrate (i.e. glycogen) metabolism, while its biochemical activity and possible factors acting downstream of PmgA are unknown. Here, a genome-wide microarray analysis of a ?pmgA strain identified the expression of 36 protein-coding genes and 42 non-coding transcripts as significantly altered. From these, the non-coding RNA Ncr0700 was identified as the transcript most strongly reduced in abundance. Ncr0700 is widely conserved among cyanobacteria. In Synechocystis its expression is inversely correlated with light intensity. Similarly to a ?pmgA mutant, a ?ncr0700 deletion strain showed an approximately 2-fold increase in glycogen content under photoautotrophic conditions and wild-Type-like growth. Moreover, its growth was arrested by 38 h after a shift to photomixotrophic conditions. Ectopic expression of Ncr0700 in ?ncr0700 and ?pmgA restored the glycogen content and photomixotrophic growth to wild-Type levels. These results indicate that Ncr0700 is required for photomixotrophic growth and the regulation of glycogen accumulation, and acts downstream of PmgA. Hence Ncr0700 is renamed here as PmgR1 for photomixotrophic growth RNA 1.

AB - Carbohydrate metabolism is a tightly regulated process in photosynthetic organisms. In the cyanobacterium Synechocystis sp. PCC 6803, the photomixotrophic growth protein A (PmgA) is involved in the regulation of glucose and storage carbohydrate (i.e. glycogen) metabolism, while its biochemical activity and possible factors acting downstream of PmgA are unknown. Here, a genome-wide microarray analysis of a ?pmgA strain identified the expression of 36 protein-coding genes and 42 non-coding transcripts as significantly altered. From these, the non-coding RNA Ncr0700 was identified as the transcript most strongly reduced in abundance. Ncr0700 is widely conserved among cyanobacteria. In Synechocystis its expression is inversely correlated with light intensity. Similarly to a ?pmgA mutant, a ?ncr0700 deletion strain showed an approximately 2-fold increase in glycogen content under photoautotrophic conditions and wild-Type-like growth. Moreover, its growth was arrested by 38 h after a shift to photomixotrophic conditions. Ectopic expression of Ncr0700 in ?ncr0700 and ?pmgA restored the glycogen content and photomixotrophic growth to wild-Type levels. These results indicate that Ncr0700 is required for photomixotrophic growth and the regulation of glycogen accumulation, and acts downstream of PmgA. Hence Ncr0700 is renamed here as PmgR1 for photomixotrophic growth RNA 1.

U2 - 10.1093/pcp/pcw128

DO - 10.1093/pcp/pcw128

M3 - Journal article

C2 - 27440548

SN - 0032-0781

VL - 57

SP - 2091

EP - 2103

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 10

ER -