The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till

TY - JOUR

T1 - The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till

AU - Bælum, Jacob

AU - Scheutz, Charlotte

AU - Chambon, Julie Claire Claudia

AU - Jensen, Christine Mosegaard

AU - Brochmann, Rikke Prejh

AU - Dennis, Philip

AU - Laier, Troels

AU - Broholm, Mette M

AU - Bjerg, Poul L

AU - Binning, Philip John

AU - Jacobsen, Carsten Suhr

N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.

PY - 2014/3

Y1 - 2014/3

N2 - A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination.

AB - A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination.

KW - Aluminum Silicates

KW - Biodegradation, Environmental

KW - DNA, Bacterial

KW - Ethylenes

KW - Halogenation

KW - Kinetics

KW - Models, Chemical

KW - Soil Microbiology

KW - Soil Pollutants

KW - Vinyl Chloride

U2 - 10.1016/j.envpol.2013.11.013

DO - 10.1016/j.envpol.2013.11.013

M3 - Journal article

C2 - 24374375

SN - 0269-7491

VL - 186

SP - 149

EP - 157

JO - Environmental Pollution

JF - Environmental Pollution

ER -