@article{e3281ba0ecb011dfb6d2000ea68e967b,
title = "Tethering of Ficolin-1 to cell surfaces through recognition of sialic acid by the fibrinogen-like domain",
abstract = "Three Ficolins have been identified in humans: Ficolin-1 (M-Ficolin), Ficolin-2 (L-Ficolin), and Ficolin-3 (H-Ficolin). Ficolin-1 is the least-described of the Ficolins and is expressed by monocytes, granulocytes, and in the lungs. Ficolin-1 is found circulating at low concentrations in serum but is regarded primarily as a secretory molecule that exerts its function locally in inflamed tissues. Ficolin-1 has been reported on the surface of monocytes and granulocytes and was suggested originally to function as a phagocytic receptor. However, the molecule does not contain any obvious transmembrane domain, and no binding partners have been identified. To gain further insight in the physiological role of Ficolin-1, we sought to identify the molecular mechanism responsible for the membrane association of Ficolin-1 to monocytes and granulocytes. We demonstrate that expression of Ficolin-1 on the cell surface is restricted to monocytes and granulocytes. Ficolin-1 is tethered to the cell surface of these cells through its fibrinogen-like domain, and the ligand involved in the binding of Ficolin-1 is shown to be sialic acid. Moreover, rFicolin-1 bound activated but not resting T lymphocytes. Together, these results demonstrate a novel self-recognition mechanism of leukocytes mediated by the fibrinogen-like domain of Ficolin-1.",
author = "Christian Honor{\'e} and Sara R{\o}rvig and Tina Hummelsh{\o}j and Mikkel-Ole Skjoedt and Niels Borregaard and Peter Garred and Christian Honor{\'e} and Sara R{\o}rvig and Tina Hummelsh{\o}j and Mikkel-Ole Skjoedt and Niels Borregaard and Peter Garred",
note = "Keywords: Calcium; Fibrinogen; Granulocytes; Humans; Lectins; Lymphocyte Activation; Monocytes; N-Acetylneuraminic Acid; Protein Structure, Tertiary; Recombinant Proteins; T-Lymphocytes; U937 Cells",
year = "2010",
month = jul,
day = "1",
doi = "10.1189/jlb.1209802",
language = "English",
volume = "88",
pages = "145--58",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "Federation of American Societies for Experimental Biology",
number = "1",
}