@article{da5f8310526511dd8d9f000ea68e967b,
title = "Target-cell-derived tRNA-like primers for reverse transcription support retroviral infection at low efficiency.",
abstract = "Reverse transcription of a retroviral genome takes place in the cytoplasm of an infected cell by a process primed by a producer-cell-derived tRNA annealed to an 18-nucleotide primer-binding site (PBS). By an assay involving primer complementation of PBS-mutated vectors we analyzed whether tRNA primers derived from the target cell can sustain reverse transcription during murine leukemia virus (MLV) infection. Transduction efficiencies were 4-5 orders of magnitude below those of comparable producer-cell complementations. However, successful usage of a target-cell-derived tRNA primer was proven by cases of correction of single mismatches between Akv-MLV vectors and complementary tRNA primers toward the primer sequence in the integrated vector. Thus, target-cell-derived tRNA-like primers are able to initiate first-strand cDNA synthesis and plus-strand transfer leading to a complete provirus, suggesting that endogenous tRNAs from the infected cell may also have access to the intracellular viral complex at that step of the replication cycle.",
author = "Alexander Schmitz and Lund, {Anders H} and Hansen, {Anette C} and Mogens Duch and Pedersen, {Finn Skou}",
note = "Keywords: 3T3 Cells; Animals; Base Sequence; Cell Line; Fibroblasts; Mice; Molecular Sequence Data; Moloney murine leukemia virus; RNA, Transfer; RNA, Transfer, Amino Acyl; RNA, Transfer, Pro; Sequence Alignment; Transcription, Genetic; Virus Replication",
year = "2002",
language = "English",
volume = "297",
pages = "68--77",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press",
number = "1",
}