TY - JOUR
T1 - Staurosporine-induced cell death in Tetrahymena thermophila has mixed characteristics of both apoptotic and autophagic degeneration
AU - Christensen, S T
AU - Chemnitz, J
AU - Straarup, E M
AU - Kristiansen, Karsten
AU - Wheatley, D N
AU - Rasmussen, L
N1 - Keywords: Animals; Apoptosis; Autophagy; Enzyme Inhibitors; Staurosporine; Tetrahymena thermophila
PY - 1998
Y1 - 1998
N2 - Staurosporine blocks signal transduction associated with cell survival, proliferation and chemosensory behaviour in the ciliated protozoan, Tetrahymena thermophila. Staurosporine inhibits cell proliferation and in vivo protein phosphorylation induced by phorbol ester. It also reduces the in vitro phosphorylation of the PKC-specific substrate, myelin basic protein fragment 4-14. Our results show that cell death in the presence of staurosporine is associated with morphological and ultrastructural changes similar to both apoptosis and autophagic degeneration, but these in turn can be postponed or prevented by 8-bromo-cyclic GMP, protoporphyrin IX, hemin or actinomycin D, although phorbol ester and insulin were ineffective. The results support the notion that staurosporine-induced cell death is an active process, associated with and/or requiring de novo RNA synthesis.
AB - Staurosporine blocks signal transduction associated with cell survival, proliferation and chemosensory behaviour in the ciliated protozoan, Tetrahymena thermophila. Staurosporine inhibits cell proliferation and in vivo protein phosphorylation induced by phorbol ester. It also reduces the in vitro phosphorylation of the PKC-specific substrate, myelin basic protein fragment 4-14. Our results show that cell death in the presence of staurosporine is associated with morphological and ultrastructural changes similar to both apoptosis and autophagic degeneration, but these in turn can be postponed or prevented by 8-bromo-cyclic GMP, protoporphyrin IX, hemin or actinomycin D, although phorbol ester and insulin were ineffective. The results support the notion that staurosporine-induced cell death is an active process, associated with and/or requiring de novo RNA synthesis.
U2 - 10.1006/cbir.1998.0320
DO - 10.1006/cbir.1998.0320
M3 - Journal article
C2 - 10452827
SN - 1065-6995
VL - 22
SP - 591
EP - 598
JO - Cell Biology International
JF - Cell Biology International
IS - 7-8
ER -