Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide

M Röpke; J Hald; Per Guldberg; J Zeuthen; L Nørgaard; L Fugger; A Svejgaard; S Van der Burg; H W Nijman; C J Melief; Mogens Helweg Claesson

Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide

M Röpke, J Hald, Per Guldberg, J Zeuthen, L Nørgaard, L Fugger, A Svejgaard, S Van der Burg, H W Nijman, C J Melief, Mogens Helweg Claesson

LUKKET: Institut for Immunologi og Mikrobiologi

145 Citationer (Scopus)

Abstract

A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.

Originalsprog	Engelsk
Tidsskrift	Proceedings of the National Academy of Sciences of the United States of America
Vol/bind	93
Udgave nummer	25
Sider (fra-til)	14704-7
Antal sider	4
ISSN	0027-8424
Status	Udgivet - 10 dec. 1996

Citationsformater

Röpke, M., Hald, J., Guldberg, P., Zeuthen, J., Nørgaard, L., Fugger, L., Svejgaard, A., Van der Burg, S., Nijman, H. W., Melief, C. J., & Claesson, M. H. (1996). Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide. Proceedings of the National Academy of Sciences of the United States of America, 93(25), 14704-7.

Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide. / Röpke, M; Hald, J; Guldberg, Per et al.

I: Proceedings of the National Academy of Sciences of the United States of America, Bind 93, Nr. 25, 10.12.1996, s. 14704-7.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Röpke, M, Hald, J, Guldberg, P, Zeuthen, J, Nørgaard, L, Fugger, L, Svejgaard, A, Van der Burg, S, Nijman, HW, Melief, CJ & Claesson, MH 1996, 'Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide', Proceedings of the National Academy of Sciences of the United States of America, bind 93, nr. 25, s. 14704-7.

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title = "Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide",

abstract = "A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.",

keywords = "Carcinoma, Squamous Cell, Cytotoxicity, Immunologic, HLA-A2 Antigen, Head and Neck Neoplasms, Humans, Mutation, T-Lymphocytes, Cytotoxic, Tumor Cells, Cultured, Tumor Suppressor Protein p53",

author = "M R{\"o}pke and J Hald and Per Guldberg and J Zeuthen and L N{\o}rgaard and L Fugger and A Svejgaard and {Van der Burg}, S and Nijman, {H W} and Melief, {C J} and Claesson, {Mogens Helweg}",

year = "1996",

month = dec,

day = "10",

language = "English",

volume = "93",

pages = "14704--7",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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TY - JOUR

T1 - Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide

AU - Röpke, M

AU - Hald, J

AU - Guldberg, Per

AU - Zeuthen, J

AU - Nørgaard, L

AU - Fugger, L

AU - Svejgaard, A

AU - Van der Burg, S

AU - Nijman, H W

AU - Melief, C J

AU - Claesson, Mogens Helweg

PY - 1996/12/10

Y1 - 1996/12/10

N2 - A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.

AB - A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.

KW - Carcinoma, Squamous Cell

KW - Cytotoxicity, Immunologic

KW - HLA-A2 Antigen

KW - Head and Neck Neoplasms

KW - Humans

KW - Mutation

KW - T-Lymphocytes, Cytotoxic

KW - Tumor Cells, Cultured

KW - Tumor Suppressor Protein p53

M3 - Journal article

C2 - 8962118

SN - 0027-8424

VL - 93

SP - 14704

EP - 14707

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 25

ER -

Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide

Abstract

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Citationsformater