Specific Mutations in Mammalian P4-ATPase ATP8A2 Catalytic Subunit Entail Differential Glycosylation of the Accessory CDC50A Subunit

TY - JOUR

T1 - Specific Mutations in Mammalian P4-ATPase ATP8A2 Catalytic Subunit Entail Differential Glycosylation of the Accessory CDC50A Subunit

AU - Vestergaard, Anna L.

AU - Mikkelsen, Stine A.

AU - Coleman, Jonathan A.

AU - Molday, Robert S.

AU - Vilsen, Bente

AU - Andersen, Jens Peter

PY - 2015/12/21

Y1 - 2015/12/21

N2 - P4-ATPases, or flippases, translocate phospholipids between the two leaflets of eukaryotic biological membranes. They are essential to the physiologically crucial phospholipid asymmetry and involved in severe diseases, but their molecular structure and mechanism are still unresolved. Here, we show that in an extensive mutational alanine screening of the mammalian flippase ATP8A2 catalytic subunit, five mutations stand out by leading to reduced glycosylation of the accessory subunit CDC50A. These mutations may disturb the interaction between the subunits.

AB - P4-ATPases, or flippases, translocate phospholipids between the two leaflets of eukaryotic biological membranes. They are essential to the physiologically crucial phospholipid asymmetry and involved in severe diseases, but their molecular structure and mechanism are still unresolved. Here, we show that in an extensive mutational alanine screening of the mammalian flippase ATP8A2 catalytic subunit, five mutations stand out by leading to reduced glycosylation of the accessory subunit CDC50A. These mutations may disturb the interaction between the subunits.

U2 - 10.1016/j.febslet.2015.11.031

DO - 10.1016/j.febslet.2015.11.031

M3 - Journal article

C2 - 26592152

SN - 0014-5793

VL - 589

SP - 3908

EP - 3914

JO - F E B S Letters

JF - F E B S Letters

IS - 24, Part B

ER -