Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide

TY - JOUR

T1 - Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide

AU - Buchardt, O

AU - Nielsen, Peter E.

PY - 1991/12/6

Y1 - 1991/12/6

N2 - A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.

AB - A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.

KW - Base Sequence

KW - Molecular Sequence Data

KW - Molecular Structure

KW - Nylons/chemistry

KW - Oligonucleotides/chemistry

KW - Photochemistry

KW - Thermodynamics

M3 - Journal article

C2 - 1962210

SN - 0036-8075

VL - 254

SP - 1497

EP - 1500

JO - Science

JF - Science

IS - 5037

ER -