Secretin uncouples glucose inhibition of glucagon-producing cells resulting in a simultaneous stimulation of both glucagon and insulin release

Hans Kofod; J J Holst; A Lernmark

Secretin uncouples glucose inhibition of glucagon-producing cells resulting in a simultaneous stimulation of both glucagon and insulin release

Hans Kofod, J J Holst, A Lernmark

Inflammation, Metabolism and Oxidation

5 Citationer (Scopus)

Abstract

The effect of secretin on glucagon and insulin release and its interaction with glucose has been studied in cultured mouse pancreatic islets by column perifusion. Glucose alone showed the well-known stimulation of insulin release and inhibition of glucagon release. Addition of 10 mM secretin increased glucagon secretion at 3 mM D-glucose by 300% while no change in insulin release could be seen at this low glucose concentration. At maximal stimulation of insulin release by 20 mM D-glucose addition of 10 nM secretin increased insulin release by 30%. Despite this insulin concentration and the high glucose concentration an increase in glucagon secretion of 1800% was found. These effects of secretin were dose-dependent at 10 mM D-glucose with 1 nM secretin being the lowest effective dose.

Originalsprog	Engelsk
Tidsskrift	Regulatory Peptides
Vol/bind	23
Udgave nummer	3
Sider (fra-til)	315-22
Antal sider	8
ISSN	0167-0115
Status	Udgivet - dec. 1988

Citationsformater

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title = "Secretin uncouples glucose inhibition of glucagon-producing cells resulting in a simultaneous stimulation of both glucagon and insulin release",

abstract = "The effect of secretin on glucagon and insulin release and its interaction with glucose has been studied in cultured mouse pancreatic islets by column perifusion. Glucose alone showed the well-known stimulation of insulin release and inhibition of glucagon release. Addition of 10 mM secretin increased glucagon secretion at 3 mM D-glucose by 300% while no change in insulin release could be seen at this low glucose concentration. At maximal stimulation of insulin release by 20 mM D-glucose addition of 10 nM secretin increased insulin release by 30%. Despite this insulin concentration and the high glucose concentration an increase in glucagon secretion of 1800% was found. These effects of secretin were dose-dependent at 10 mM D-glucose with 1 nM secretin being the lowest effective dose.",

keywords = "Animals, Cells, Cultured, Dose-Response Relationship, Drug, Glucagon, Glucose, Insulin, Islets of Langerhans, Male, Mice, Secretin",

author = "Hans Kofod and Holst, {J J} and A Lernmark",

year = "1988",

month = dec,

language = "English",

volume = "23",

pages = "315--22",

journal = "Regulatory Peptides",

issn = "0167-0115",

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TY - JOUR

T1 - Secretin uncouples glucose inhibition of glucagon-producing cells resulting in a simultaneous stimulation of both glucagon and insulin release

AU - Kofod, Hans

AU - Holst, J J

AU - Lernmark, A

PY - 1988/12

Y1 - 1988/12

N2 - The effect of secretin on glucagon and insulin release and its interaction with glucose has been studied in cultured mouse pancreatic islets by column perifusion. Glucose alone showed the well-known stimulation of insulin release and inhibition of glucagon release. Addition of 10 mM secretin increased glucagon secretion at 3 mM D-glucose by 300% while no change in insulin release could be seen at this low glucose concentration. At maximal stimulation of insulin release by 20 mM D-glucose addition of 10 nM secretin increased insulin release by 30%. Despite this insulin concentration and the high glucose concentration an increase in glucagon secretion of 1800% was found. These effects of secretin were dose-dependent at 10 mM D-glucose with 1 nM secretin being the lowest effective dose.

AB - The effect of secretin on glucagon and insulin release and its interaction with glucose has been studied in cultured mouse pancreatic islets by column perifusion. Glucose alone showed the well-known stimulation of insulin release and inhibition of glucagon release. Addition of 10 mM secretin increased glucagon secretion at 3 mM D-glucose by 300% while no change in insulin release could be seen at this low glucose concentration. At maximal stimulation of insulin release by 20 mM D-glucose addition of 10 nM secretin increased insulin release by 30%. Despite this insulin concentration and the high glucose concentration an increase in glucagon secretion of 1800% was found. These effects of secretin were dose-dependent at 10 mM D-glucose with 1 nM secretin being the lowest effective dose.

KW - Animals

KW - Cells, Cultured

KW - Dose-Response Relationship, Drug

KW - Glucagon

KW - Glucose

KW - Insulin

KW - Islets of Langerhans

KW - Male

KW - Mice

KW - Secretin

M3 - Journal article

C2 - 3070646

SN - 0167-0115

VL - 23

SP - 315

EP - 322

JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 3

ER -

Secretin uncouples glucose inhibition of glucagon-producing cells resulting in a simultaneous stimulation of both glucagon and insulin release

Abstract

Fingeraftryk

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