Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection

TY - JOUR

T1 - Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection

AU - Dahly, Elizabeth M

AU - Gillingham, Melanie B

AU - Guo, Ziwen

AU - Murali, Sangita G

AU - Nelson, David W

AU - Holst, Jens Juul

AU - Ney, Denise M

PY - 2003/4

Y1 - 2003/4

N2 - To elucidate the role of luminal nutrients and glucagon-like peptide-2 (GLP-2) in intestinal adaptation, rats were subjected to 70% midjejunoileal resection or ileal transection and were maintained with total parenteral nutrition (TPN) or oral feeding. TPN rats showed small bowel mucosal hyperplasia at 8 h through 7 days after resection, demonstrating that exogenous luminal nutrients are not essential for resection-induced adaptation when residual ileum and colon are present. Increased enterocyte proliferation was a stronger determinant of resection-induced mucosal growth in orally fed animals, whereas decreased apoptosis showed a greater effect in TPN animals. Resection induced significant transient increases in plasma bioactive GLP-2 during TPN, whereas resection induced sustained increases in plasma GLP-2 during oral feeding. Resection-induced adaptive growth in TPN and orally fed rats was associated with a significant positive correlation between increases in plasma bioactive GLP-2 and proglucagon mRNA expression in the colon of TPN rats and ileum of orally fed rats. These data support a significant role for endogenous GLP-2 in the adaptive response to mid-small bowel resection in both TPN and orally fed rats.

AB - To elucidate the role of luminal nutrients and glucagon-like peptide-2 (GLP-2) in intestinal adaptation, rats were subjected to 70% midjejunoileal resection or ileal transection and were maintained with total parenteral nutrition (TPN) or oral feeding. TPN rats showed small bowel mucosal hyperplasia at 8 h through 7 days after resection, demonstrating that exogenous luminal nutrients are not essential for resection-induced adaptation when residual ileum and colon are present. Increased enterocyte proliferation was a stronger determinant of resection-induced mucosal growth in orally fed animals, whereas decreased apoptosis showed a greater effect in TPN animals. Resection induced significant transient increases in plasma bioactive GLP-2 during TPN, whereas resection induced sustained increases in plasma GLP-2 during oral feeding. Resection-induced adaptive growth in TPN and orally fed rats was associated with a significant positive correlation between increases in plasma bioactive GLP-2 and proglucagon mRNA expression in the colon of TPN rats and ileum of orally fed rats. These data support a significant role for endogenous GLP-2 in the adaptive response to mid-small bowel resection in both TPN and orally fed rats.

KW - Adaptation, Physiological

KW - Animals

KW - Apoptosis

KW - Body Weight

KW - Cell Division

KW - Eating

KW - Enterocytes

KW - Glucagon

KW - Glucagon-Like Peptide 2

KW - Glucagon-Like Peptides

KW - Ileum

KW - Jejunum

KW - Male

KW - Parenteral Nutrition

KW - Peptides

KW - Proglucagon

KW - Protein Precursors

KW - Rats

KW - Rats, Sprague-Dawley

KW - Sucrase

U2 - 10.1152/ajpgi.00293.2002

DO - 10.1152/ajpgi.00293.2002

M3 - Journal article

C2 - 12505881

SN - 0193-1857

VL - 284

SP - G670-82

JO - American Journal of Physiology: Gastrointestinal and Liver Physiology

JF - American Journal of Physiology: Gastrointestinal and Liver Physiology

IS - 4

ER -