Rheumatoid factor and its interference with cytokine measurements: problems and solutions

Else Marie Bartels; Inger Falbe Wätjen; Eva Littrup Andersen; Bente Danneskiold-Samsøe; Henning Bliddal; Søren Ribel-Madsen

doi:http://dx.doi.org/10.1155/2011/741071

Rheumatoid factor and its interference with cytokine measurements: problems and solutions

Else Marie Bartels, Inger Falbe Wätjen, Eva Littrup Andersen, Bente Danneskiold-Samsøe, Henning Bliddal, Søren Ribel-Madsen

Abstract

Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65-80% of RA patients. Partly removal of RF is possible by precipitation. This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems. IL-1ß, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation. Presence of RF does interfere with MIA. PEG 6000 precipitation abolishes this RF interference. We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients.

Originalsprog	Engelsk
Tidsskrift	Arthritis Today
Vol/bind	2011
Sider (fra-til)	741071
ISSN	0969-7039
DOI	https://doi.org/10.1155/2011/741071
Status	Udgivet - 2011

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http://dx.doi.org/10.1155/2011/741071

Citationsformater

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title = "Rheumatoid factor and its interference with cytokine measurements: problems and solutions",

abstract = "Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65-80% of RA patients. Partly removal of RF is possible by precipitation. This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems. IL-1{\ss}, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation. Presence of RF does interfere with MIA. PEG 6000 precipitation abolishes this RF interference. We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients.",

author = "Bartels, {Else Marie} and {Falbe W{\"a}tjen}, Inger and {Littrup Andersen}, Eva and Bente Danneskiold-Sams{\o}e and Henning Bliddal and S{\o}ren Ribel-Madsen",

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TY - JOUR

T1 - Rheumatoid factor and its interference with cytokine measurements

T2 - problems and solutions

AU - Bartels, Else Marie

AU - Falbe Wätjen, Inger

AU - Littrup Andersen, Eva

AU - Danneskiold-Samsøe, Bente

AU - Bliddal, Henning

AU - Ribel-Madsen, Søren

PY - 2011

Y1 - 2011

N2 - Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65-80% of RA patients. Partly removal of RF is possible by precipitation. This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems. IL-1ß, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation. Presence of RF does interfere with MIA. PEG 6000 precipitation abolishes this RF interference. We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients.

AB - Use of cytokines as biomarkers for disease is getting more widespread. Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems. In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic. RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65-80% of RA patients. Partly removal of RF is possible by precipitation. This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems. IL-1ß, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation. Presence of RF does interfere with MIA. PEG 6000 precipitation abolishes this RF interference. We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients.

U2 - http://dx.doi.org/10.1155/2011/741071

DO - http://dx.doi.org/10.1155/2011/741071

M3 - Journal article

SN - 0969-7039

VL - 2011

SP - 741071

JO - Arthritis Today

JF - Arthritis Today

ER -

Rheumatoid factor and its interference with cytokine measurements: problems and solutions

Abstract

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